- Research article
- Open Access
Feed restriction and a diet's caloric value: The influence on the aerobic and anaerobic capacity of rats
Journal of the International Society of Sports Nutritionvolume 9, Article number: 10 (2012)
The influence of feed restriction and different diet's caloric value on the aerobic and anaerobic capacity is unclear in the literature. Thus, the objectives of this study were to determine the possible influences of two diets with different caloric values and the influence of feed restriction on the aerobic (anaerobic threshold: AT) and anaerobic (time to exhaustion: Tlim) variables measured by a lactate minimum test (LM) in rats.
We used 40 adult Wistar rats. The animals were divided into four groups: ad libitum commercial Purina® diet (3028.0 Kcal/kg) (ALP), restricted commercial Purina® diet (RAP), ad libitum semi-purified AIN-93 diet (3802.7 Kcal/kg) (ALD) and restricted semi-purified AIN-93 diet (RAD). The animals performed LM at the end of the experiment, 48 h before euthanasia. Comparisons between groups were performed by analysis of variance (p < 0,05).
At the end of the experiment, the weights of the rats in the groups with the restricted diets were significantly lower than those in the groups with ad libitum diet intakes. In addition, the ALD group had higher amounts of adipose tissue. With respect to energetic substrates, the groups subjected to diet restriction had significantly higher levels of liver and muscle glycogen. There were no differences between the groups with respect to AT; however, the ALD group had lower lactatemia at the AT intensity and higher Tlim than the other groups.
We conclude that dietary restriction induces changes in energetic substrates and that ad libitum intake of a semi-purified AIN-93 diet results in an increase in adipose tissue, likely reducing the density of the animals in water and favouring their performance during the swimming exercises.
Several authors have studied the effects of caloric restriction on body composition and metabolic variables in both humans [1–3] and animals . Reducing daily feed intake to 20 to 40% below ad libitum levels, or providing feed intermittently rather than continuously, has been found to significantly reduce the risk of chronic degenerative diseases such as cancer, type-II diabetes and kidney diseases, and to prolong the life span of laboratory rats and mice by 40% without causing malnutrition [4–7].
However, excessive dietary restriction can lead to malnutrition and physiological changes that lead to decreases in sympathetic nervous system activity, changes in thyroid metabolism, reductions in insulin concentrations and changes in glucagon, growth hormone and glucocorticoid secretion . Furthermore, these changes may promote the mobilisation of endogenous substrates, leading to increased circulation of fatty acids and increased protein catabolism (including a reduction in muscle protein - ), reflecting the decrease in energy expenditures .
According to Vanittalie and Yang , additional changes may occur to the protein content of heart muscle fibres. Individuals who have lost a significant amount of weight (30% of initial weight) have reduced cardiac mass, and heart muscle fibre atrophy occurs when dietary restriction is implemented in excess, thus reducing the vital capacity of individuals and potentially impairing aerobic and anaerobic performance. These changes, which occur because of an energy deficit, may lead to vital changes in the body.
Given the limitations on human research, animal models have become very important tools for studying many areas of science, including exercise physiology. The use of overweight and inactive animals as controls can affect the results of studies. Therefore, researchers are currently proposing that new procedures for maintaining laboratory animals be evaluated and implemented in order to assure that the control animals are fed in portions rather than ad libitum, and that they have adequate environmental stimulation and physical activity .
The use of a "control diet" is fundamentally important to researchers. Two types of "control diets" are frequently used in physiological studies: a commercial rodent diet (Purina®) and a diet proposed by the American Institute of Nutrition in 1993 (AIN-93) . However, these two diets have different caloric contents (Purina®: 3028.0 Kcal/kg and American Institute of Nutrition diet (AIN-93 M): 3802.7 Kcal/kg) that are not usually considered. Moreover, it is important to determine the possible effects of the feeding protocol and the different diets fed to rodents that are used in exercise physiology studies.
The lactate minimum test has been used in exercise physiology studies of both humans [13–16] and rodents [17–19] because it enables researchers to determine aerobic and anaerobic capabilities in a single test [20, 21]. However, the possible effects of administering diets with different caloric values and feed restriction on the parameters provided by the lactate minimum test are not well understood in the literature. Therefore, the purpose of this study was to evaluate the effects of dietary restriction (60% of ad libitum intake) of two control diets (commercial Purina® and American Institute of Nutrition diet (AIN-93 M)) on the aerobic and anaerobic capacity of Wistar rats, as determined by the lactate minimum test.
Animals and animal care
The duration of study was one month and we used 40 Wistar rats that were from 90 days old at the beginning of the experiment and had body weights of 406.9 ± 39.44 g. The animals were housed in polyethylene cages measuring 37 × 31 × 16 cm (five rats per cage) at room temperature (25°C) with a 12-hour light/dark photoperiod. All procedures involving animals were submitted to and approved by the Ethics Committee on Animal Use in Research of the Biosciences Institute of UNESP, Rio Claro Campus (protocol number: 2011/6274).
The animals were divided into four groups with 10 animals per group, depending on the diet and mode of administration. Two groups had access to commercial feed (Purina®): one ad libitum (ALP) and the other restricted (RAP). The two other groups had access to the diet proposed by the American Institute of Nutrition in 1993 (AIN-93 M): one ad libitum (ALD) and the other restricted (RAD). Feed intake for the animals in the ad libitum groups was recorded daily. Thus, for the animals on feed restriction, feed was offered in an amount corresponding to 60% of the average amount consumed by the ad libitum groups the previous day. This protocol was selected to allow for dietary restriction without causing malnutrition . All groups had free access to water.
Commercial Purina ® Diet (Paulínia/SP, Brazil)
This diet was composed of 43.7% carbohydrates, 23% protein, and 4% fat at 3,028 kcal/g. The remainder of the ingredients were comprised of minerals, fibre, and vitamins.
AIN-93M (Semi-purified diet, according to the American Institute of Nutrition, AIN-93M; )
The diet was composed of 70% carbohydrates, 14% protein, and 4% fat at 3,802.7 kcal/g. The remainder of the ingredients were comprised of minerals, fibre, and vitamins.
Adaptation to water
Before undergoing the lactate minimum protocol, all the animals were adapted only one time to water. The adaptation occurred over a total period of five continuous days, by placing the animals in shallow water in the tank where the tests occurred. The water temperature was maintained at 31 ± 1°C . The purpose of the adaptation was to reduce the stress of the animals, without promoting physiological adaptations that result from physical training.
Evaluation of aerobic and anaerobic capacity
To determine acutely aerobic and anaerobic capacity, we used the lactate minimum test, which enabled us to determine both parameters in a single protocol [20, 21]. This test consists of an induction phase to hyperlactatemia (anaerobic exercise) followed by progressive exercise.
The induction phase consisted of two efforts with a load equivalent to 13% of the animals' body weight. The first effort lasted 30 s, followed by a 30-s passive recovery period. After the recovery period, the animals performed a maximum effort to obtain the time to exhaustion, considered as the parameter of anaerobic fitness. Nine minutes after the exhaustion period, we collected 25 μl of blood via a cut at the distal end of the tail to determine lactate concentrations.
After collecting the blood, the animals began a progressive phase with an initial intensity of 4.0% of body weight, which was increased by increments of 0.5% of body weight over 5 min intervals. At the end of each stage, 25 μl of blood was collected to determine lactate concentrations. The anaerobic threshold, considered as the parameter for aerobic capacity, was equivalent to the zero derivative of a second-order polynomial fit that was obtained from the relationship between lactate concentrations and the exercise intensity. Consequently, we determined lactate concentrations based on the anaerobic threshold.
During all the efforts, the animals were placed individually in tanks (100 × 80 × 80 cm) containing water at 31 ± 1°C. Blood samples were collected using calibrated capillary tubes and heparinised, and blood lactate was determined using an enzymatic method .
Evaluations conducted during the intervention and before euthanasia
Throughout the experimental period, the body weights (all groups) and feed intakes (ad libitum group) were recorded daily using an analytical balance. The results were analysed based on the weight change of the animals (weight change = initial weight - final weight).
Parameters obtained following euthanasia
At the end of the experiment, animals were anesthetised in a CO2 chamber, 48 h after measuring the lactate minimum test. Blood was collected directly from the heart via cardiac puncture with disposable needles and syringes. The blood was subsequently centrifuged at 3000 rpm for 15 m, and the serum supernatant was used to determine glucose, total protein and albumin content  using commercially available colorimetric enzymatic kits (Labor-lab, Brazil).
Samples of the gastrocnemius (red and white portions) and soleus muscles were collected and used to assess glycogen  and triglyceride content . We also collected liver samples for glycogen  and total lipid analyses . All the samples were homogenised in a Polytron® for 20 s at maximum speed. They were then centrifuged at 10,000 rpm for 5 min at 4°C prior to the analyses.
The normality of the data was confirmed using the Shapiro-Wilk test. The results are presented as the mean ± standard deviation. Comparisons between groups were performed by analysis of variance (one-way ANOVA) and the Newman-Keuls Post-hoc test when necessary. For all the analyses, the level of significance was set at p < 0.05 (Statistica 7; Statsoft, USA).
During the interventions in this study, the animals from the RAP and RAD groups showed a significant decrease in body weight over the course of the experimental period (Figure 1). However, neither group showed any clinical indications of malnutrition, such as hypoalbuminemia, hypoproteinemia or high lipid content in the liver (LIPLIV).
Nevertheless, animals in the RAD group had significantly lower LIPLIV compared to the ALP and ALD groups (p < 0.05) (Table 1). The change in weight during the intervention (weight change = initial weight - final weight) was significantly higher for the ALD group compared to the ALP group (Figure 2). Furthermore, the ALD group had greater amounts of subcutaneous adipose tissue (p < 0.05) than the other groups. In contrast, the RAP and RAD groups had significantly less adipose tissue in the mesenteric and retroperitoneal regions compared to the ad libitum groups (Table 2).
The levels of liver glycogen (GLYCLIV) in the RAD and RAP groups were significantly higher (p < 0.05) than those found in the ALP and ALD groups. Moreover, the quantities of soleus muscle glycogen (GLYCSOL) in the RAP group were also higher than in the ALP and ALD groups (p < 0.05). There were no significant differences between the groups with respect to the levels of triglycerides found in the soleus (TGSOL) and gastrocnemius (TGGAS) muscles (Table 3).
Table 4 shows the values for aerobic capacity, lactate concentrations and anaerobic capacity (time to exhaustion) determined using the lactate minimum test in all the groups studied. The anaerobic threshold values did not differ between the groups, whereas the lactate concentrations values were significantly lower (p < 0.05) in the ALD group compared to other groups. In addition, the ALD group had higher time to exhaustion (p < 0.05) compared to the ALP and RAP groups (Table 4).
The principle findings of this study demonstrate that a 40% restriction on the amount of feed offered to the rats did not cause malnutrition in adult Wistar rats over a four-week period. In addition, the caloric difference between the two control diets used (Purina®: 3028.0 Kcal/kg and AIN-93 M: 3802.7 Kcal/kg) did not cause changes in the levels of muscle and liver glycogen, whereas the way in which the diets were administered resulted in increased levels of these substrates in the animals in the RAP and RAD groups. Additionally, the American Institute of Nutrition diet (AIN-93 M) that was administered ad libitum improved the aerobic and anaerobic capacity of the ALD group, probably due to the lower density of these animals in water.
Malnutrition in animals is often characterised by low serum albumin and total protein concentrations and high levels of liver lipids [18, 25]. In the present study, the animals that had restricted access to feed (RAP and RAD) did not show these characteristics, confirming previous research . In addition, studies have shown that dietary restriction (80 to 60% of ad libitum intake) decreases the risk of chronic degenerative diseases such as cancer, type-2 diabetes and kidney disease, prolonging the life span of laboratory rats and mice by up to 40% without causing malnutrition [5–7].
Comparing the effects of a standard diet (Purina®) to those of a freely administered high calorie diet, Chun, Lee, Kim, et al.  showed that animals on a high calorie diet have higher levels of body fat. These findings are consistent with the present study, where the ALD group, which was fed a higher caloric diet American Institute of Nutrition diet (AIN-93 M), showed more weight gain than the ALP group. According to Silva, Marcondes and Mello , animals that are subjected to high-fat diets tend to accumulate more fat than control animals.
The RAP and RAD groups showed higher glycogen values, primarily in the soleus muscle and liver, than those fed ad libitum. Corroborating these findings, Pedrosa, Tirapegui, Rogero, et al. , when comparing sedentary and trained animals, both with and without feed restriction (25 and 50% of ad libitum intake), observed higher muscle and liver glycogen values in the animals in the restricted groups. In addition, Wetter, Gazdag, Dean, et al.  observed similar or higher muscle glycogen values in rats subjected to feed restriction (60% of consumed by the ad libitum group), as has been demonstrated by other studies [30, 31]. Conversely, these authors found higher liver glycogen values in animals fed ad libitum, suggesting that the influence of dietary restriction on the content of this substrate is dependent on the tissue analysed. In this regard, further studies are needed to determine the changes caused by dietary restriction on the mechanisms of glycogen synthesis and utilisation in different tissues.
The differences in the levels of muscular glycogen could influence aerobic and anaerobic capacity in animals, as determined using the lactate minimum test. However, there were no significant differences in the anaerobic threshold values between the groups, demonstrating that the diets and their form of administration did not influence the aerobic capacity of the animals. In addition, the loads corresponding to the anaerobic threshold in relation to body weight (4.5%) are similar to those reported by previous studies that used eutrophic rats [18, 32, 33] ARAÚJO et al., 2007).
However, the animals in the ALD group showed lower lactate concentrations values. This finding, together with the lower quantities of glycogen in the ad libitum groups, is consistent with those reported by Voltarelli, Gobatto and Mello , who observed lower lactate concentrations values in glycogen-depleted animals when comparing anaerobic threshold determined using lactate minimum test in a group of fed animals and a group of animals subjected to glycogen depletion.
The animals in the ALD group showed the same characteristics observed in humans subjects during a lactate minimum test after glycogen depletion, i.e., the intensity corresponding to the minimum lactate concentration was not influenced by a reduction in glycogen stores; however, the lactate concentrations were significantly lower upon depletion . Further, the lactate concentrations and time to exhaustion values may have been influenced by the density of the animals in the ALD group, since these animals had an increase in body weight and body fat.
Araújo, Araújo, Dangelo, et al.  demonstrated that the anaerobic threshold in obese animals, as determined using maximal steady state lactate levels, may be higher than that in well-nourished animals, attributing these findings to the lower density of these animals in an aquatic environment. Thus, in our study, the intensity at the same workload may have been underestimated for animals that have higher levels of fat , resulting in the lower lactate concentrations values and higher time to exhaustion values seen in the ALD group. Therefore, more studies are needed to normalise the variables related to the increased loads used in lactateminimum test as a function of the body density of the animals.
The results of this study suggest that the caloric differences between the two diets did not noticeably influence the levels of muscle and liver glycogen, whereas these levels could be influenced by the form in which the diets were administered. However, the higher levels of glycogen seen in the RAP and RAD groups did not influence the aerobic and anaerobic capacity as determined using the lactate minimum test. In addition, the lower lactate concentrations and higher time to exhaustion values seen for the ALD group may be explained by the lower density of the animals in this group. Thus, one limitation of this study was the lack of quantification of the density of the animals and the use of loads that did not consider this variable in water.
In summary, feed restriction induced changes in energetic substrates, and ad libitum intake of a semi-purified American Institute of Nutrition diet (AIN-93 M) resulted in increased adipose tissue, which likely reduced the density of the animals in water and favoured their performance in the swimming exercise.
From the results of this study, we can conclude that: 1) the animals in the diet-restricted groups showed no manifestations of malnutrition, indicating that the amount of feed offered (60% of that consumed by the ad libitum group) was sufficient; 2) the caloric differences in the diets studied did not alter the levels of muscle and liver glycogen, whereas the form of administration (ad libitum or restricted) did modify the quantities of these substrates; 3) the differences in the levels of glycogen between the two groups had little influence on the aerobic and anaerobic capacity of the animals; and 4) the ALD group animals may have had a lower density in water, which might have influenced the lactate concentrations and time to exhaustion values observed in this group.
Time to Exhaustion
Lactate Minimum Test
Ad libitum commercial (Purina®) diet group
Restricted commercial (Purina®) diet group
Diet proposed by the American Institute of Nutrition in 1993
Ad libitum semi-purified AIN-93 diet group
Restricted semi-purified AIN-93 diet group.
Lactate concentrations at Anaerobic Threshold intensity.
Concentrations of albumin
Lipid content in the liver
Glycogen content in the liver
Soleus muscle glycogen content
Triglycerides content in the soleus muscle
Triglycerides content in the gastrocnemius muscle.
Yu BP, Masoro EJ, Murata I, Bertrand HA, Lynd FT: Life Span Study of SPF Fischer 344 Male Rats Fed AdLibitum or Restricted Diets: Longevity, Growth, Lean Body Mass and Disease. J Gerontol. 1982, 2: 130-141.
Oscai LB, Holloszy JO: Effects of weight changes produced by exercise, food restriction, or overeating on body composition. J Clin Invest. 1969, 48: 2124-2128. 10.1172/JCI106179.
Holloszy JO: Exercise increases average longevity of female rats despite increased food intake and no growth retardation. J Gerontol. 1993, 48: 97-100.
Weindruch R, Walford RL, Fligiel S, Guthrie D: The Retardation of Aging in Mice by Dietary Restriction: Longevity, Cancer, Immunity and Lifetime Energy Intake. J Nutr. 1986, 116: 641-654.
Weindruch R: The retardation of aging by caloric restriction: Studies in rodents and primates. Toxicol Pathol. 1996, 24: 742-745. 10.1177/019262339602400618.
Mattson MP, Wan R: Beneficial effects of intermittent fasting and caloric restriction on the cardiovascular and cerebrovascular systems. J Nutr Biochem. 2005, 16: 129-137. 10.1016/j.jnutbio.2004.12.007.
Heilbronn LK, Ravussin E: Calorie restriction and aging: review of the literature and implications for studies in humans. Am J Clin Nutr. 2003, 78: 361-369.
Shetty PS: Physiological mechanisms in the adaptive response of metabolic rates to energy restriction. Nutr Res Rev. 1990, 3: 49-74. 10.1079/NRR19900006.
Walberg JL: Aerobic exercise and resistance weight training during weight reduction. Implications for obese persons and athletes. Sports Med. 1989, 7: 343-356. 10.2165/00007256-198907060-00001.
Vanitallie TB, Yang M: Cardiac dysfunction in obese dieters: a potentially lethal complication of rapid, massive weight loss. Am J Clin Nutr. 1984, 39: 695-702.
Martin B, Ji S, Stuart MS, Mattson MP: "Control" laboratory rodents are metabolically morbid: Why it matters. PNAS. 2010, 107: 6127-33. 10.1073/pnas.0912955107. (EarlyEdition)
Reeves PG, Nielsen FH, Fahey GC: AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993, 11: 1939-1951.
Knoepfli-lenzin C, Boutellier U: Lactate Minimum is Valid to Estimate Maximal Lactate Steady State in Moderately and Highly Trained Subjects. J Strength Condit Res. 2011, 5: 1355-1359.
Dotan R, Zigel L, Rotstein A, Greenberg T, Benyamini Y, Falk B: Reliability and validity of the lactate-minimum test. A revisit. J Sports Med Phys Fitness. 2011, 1: 42-49.
Pardono E, Madrid B, Motta DF, Mota MR, Campbell CSG, Simões HG: Lactato mínimo em protocolo de rampa e sua validade em estimar o máximo estado estável de lactato. Rev Bras Cineantropometria Desempenho Hum. 2009, 2: 174-180.
Souza TNT, Yamaguti SAL, Campbell CSG, Simões HG: Identificação do lactato mínimo e glicose mínima em indivíduos fisicamente ativos. R Bras Ci e Mov Brasília. 2003, 2: 71-75.
Oliveira CA, Paiva MF, Mota CA, Ribeiro C, Leme JA, Luciano E, Mello MA: Exercise at anaerobic threshold intensity and insulin secretion by isolated pancreatic islets of rats. Islets. 2010, 4: 240-246.
Voltarelli FA, Gobatto CA, Mello MAR: Determinação da transição metabólica através do teste do lactato mínimo em ratos desnutridos durante o exercício de natação. R da Educação Física. 2007, 1: 33-39.
Gobatto CA, Mello MAR, Sibuya CY, Azevedo JRM, Santos LA, Kokubon E: Maximal lactate steady state in rats submitted to swimming exercise. Comp Biochem Physiol. 2001, 130: 21-27. 10.1016/S1095-6433(01)00362-2.
Tegtbur U, Busse MW, Braumann KM: Estimation of individual equilibrium between production and catabolism curing exercise. Med Sci Sports Exerc. 1993, 5: 620-627.
de Araujo GG, Papoti M, de Manchado FB, de Mello MA, Gobatto CA: Protocols for hyperlactacidemia induction in lactate minimum test adapted to swimming rats. Comp Biochem Physiol. 2007, 4: 888-892.
Engels RC, Jones JB: Causes and elimination of erratic blanc in enzymatic metabolic assays involving the use of NAD in alkaline hydrazine buffers: improved conditions for assay of L-glutamate. L-lactate and other metabolites. Anal Biochem. 1978, 88: 475-484. 10.1016/0003-2697(78)90447-5.
Nogueira DM: Sangue-parte I, et al: Glicídios. Métodos de bioquímica clínica. Edited by: Nogueira DM, et al. 1990, São Paulo: Pancast, 153-168.
Lo S, Russeau JC, Taylor AW: Determination of glycogen in small tissue samples. J Appl Physiol. 1970, 2: 234-236.
Almeida PBL, Mello MAR: Desnutrição protéica fetal/neonatal, ação da insulina e homeostase glicêmica na vida adulta: efeitos do jejum e do exercício agudo. Rev Bras Educação Física. 2004, 1: 17-30.
Chun MR, Lee YJ, Kim KH, Kim YW, Park SY, Lee KM, Kim JY, Park YK: Differential effects of high-carbohydrate and high-fat diet composition on muscle insulin resistance in rats. J Korean Med Sci. 2010, 7: 1053-1059.
Silva MPD, Marcondes MCCG, Mello MAR: Exercício aeróbio e anaeróbio: Efeitos sobre a gordura sérica e tecidual de ratos alimentados com dieta hiperlipídica. Rev Bras Atividade Física e Saúde. 1999, 3: 43-56.
Pedrosa RG, Tirapegui J, Rogero MM, Castro IA, Pires ISO, Oliveira AAM: Influência do exercício físico na composição química da massa corporal magra de ratos submetidos à restrição alimentar. Revista Brasileira de Ciências Farmacêuticas. 2004, 1: 27-34.
Wetter TJ, Gazdag AC, Dean DJ, Cartee GD: Effect of calorie restriction on in vivo glucose metabolism by individual tissues in rats. Am J Physiol. 1999, 276: 728-738.
Gupta G, She L, Ma XH, Yang XM, Hu M, Cases JA, Vuguin P, Rossetti L, Barzilai N: Aging does not contribute to the decline in insulin action on storage of muscle glycogen in rats. Am J Physiol Regul Integr Comp Physiol. 2000, 278: 111-117.
Montori-Grau M, Minor R, Lerin C, Allard J, Garcia-Martinez C, de Cabo R, Gómez-Foix AM: Effects of aging and calorie restriction on rat skeletal muscle glycogen synthase and glycogen phosphorylase. Exp Gerontol. 2009, 6-7: 426-433.
Voltarelli FA, Gobatto CA, Mello MAR: Determination of anaerobic threshold in rats using the lactate minimum test. Braz J Med Biol Res. 2002, 35: 1389-1394.
Voltarelli FA, Gobatto CA, Mello MAR: Glicogênio muscular e limiar anaeróbio determinado em ratos durante a natação. Motriz. 2004, 1: 25-30.
de Araujo GG, Araujo MB, Dangelo RA, Machado FB, Mota CSA, Ribeiro C, Mello MAR: Máxima Fase Estável de Lactato em Ratos Obesos de Ambos os Gêneros. Rev Bras Med Esporte. 2009, 1: 46-49.
Voltarelli FA, Nunes WMS, Santiago V, Pauli JR, Garcia DR, Romero C, Silva AS, Mello MAR: Determinação do Limiar Anaeróbio em Ratas Obesas com Glutamato Monossódico (MSG). Revista Logos. 2003, 11: 84-93.
The authors would like to thank the technicians at the Biodynamic Laboratory of the Physical Education Department at UNESP Campus Rio Claro for their indispensible support, Clarice Sibuya and José Roberto Rodrigues, and the National Council of Scientific and Technological Development - CNPq, the Foundation for Research Support of São Paulo - FAPESP for the financial support and FUNDUNESP. We also thank Corn Products Brasil® for the donation of the dietary materials used in this experiment.
The authors declare that they have no competing interests.
All authors were responsible for the experimental design, data collection, statistical analysis and preparation of the manuscript. All authors worked read and approved the final manuscript.