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Impact of cow’s milk intake on exercise performance and recovery of muscle function: a systematic review


Dairy products are thought to improve recovery after both resistance and endurance exercises due to their nutritional proprieties. We systematically reviewed the effects of dairy product intake on exercise performance and recovery of muscle function in humans. A literature search was conducted in the MEDLINE (via PubMed) and Web of Science databases from their inception to 15th April 2018. The initial search retrieved 7708 articles, and a total of 11 studies were finally included after applying inclusion and exclusion criteria. All the selected studies were conducted with cow’s milk. Whereas some studies found significant positive effect of cow’s milk on exercise performance and recovery of muscle function, others did not find any effect. These controversies could be due to the heterogeneity of cow’s milk ingestion (e.g., amount of cow’s milk, timing of consuming the cow’s milk), to the type of intervention, and to the large heterogeneity of outcomes measured. Limited studies exist examining the effects of cow’s milk consumption and its influence on exercise performance and recovery of muscle function, therefore further studies are needed to draw more definitive conclusions.


There is evidence that proper nutritional intake is a key factor in optimizing exercise performance as well as adaptation to training (e.g., positive stimuli for protein synthesis in skeletal muscle) and recovery of muscle function (e.g. increase the recovery between training sessions or competitions, decrease the symptoms of delayed onset muscle soreness, etc.) [1, 2]. High exercise performance requires very controlled nutritional intake [3] and timing [4] before, during and after exercise to maximize exercise-induced adaptation and to shorten recovery after exercise, however the impact of either the type, composition or timing of the nutrient is still not known. Protein intake has a great impact on muscle damage repair, facilitating the recovery of muscle function (e.g. muscle strength, muscular power production, muscular stiffness, etc.) and muscular protein synthesis [1, 2]. For both hypertrophy and recovery, a positive muscle protein net balance, i.e., a higher muscle protein synthesis than muscle protein breakdown, is necessary [5].

When the rates of muscle protein synthesis and degradation increase [6, 7], an adequate nutrition is required [8,9,10] to facilitate the recovery process. For example, a bout of unaccustomed exercise, especially that including eccentric muscle contractions such as downhill running, can damage contractile proteins, impair muscle function, and induce muscle soreness [11, 12]. In theory, the stimulation of muscle protein synthesis by means of protein or amino acids (e.g. through dairy products ingestion) represents an important skeletal muscle adaptive response to mechanical stress that helps in recovery of muscle function [8, 13, 14].

Dairy products are rich in amino acids, proteins, lipids, minerals and vitamins, and their health benefits have been reviewed elsewhere [15]. These beneficial properties are based on the fact that dairy products, and especially cow’s milk, contains lactose (carbohydrate), casein and whey protein—commonly in a 3:1 ratio (casein:whey), as well as calcium [5, 15]. Of note is that these other nutrients present in cow’s milk such as calcium, sodium or potassium could aid in fluid recovery after exercising [5] and this improvement in the hydration state could help the recovery of the skeletal muscle. Furthermore, the aforementioned protein ratio could promote slow digestion and absorption of amino acids [5], which may lead to an increase in the serum amino acid concentration (mainly branched amino acids) [16], however, it is important to note that casein alone or whey protein alone, could increase serum amino acid concentration. These branched amino acids may have a large impact on protein synthesis and muscle metabolism [5] and therefore, helping the aforementioned muscle damage repair process. However, it is important to note that Atherton et al. [17] showed that branched amino acids effect on muscular protein synthesis is most likely due to the presence of leucine and not the presence of isoleucine or valine. Furthermore Witard et al. [18] reported that muscular protein synthesis stimulation via branched amino acids was ~ 50% inferior compared to a whey protein bolus containing similar amounts of branched amino acids. Moreover, the nutritional characteristics of dairy products (e.g. cow’s milk) [19] plus the relatively low price and high availability [20] of dairy products make them a potentially recovery-enhancing product after exercise [5]. This is observed in the current growth of scientific interest in the effects of dairy product intake on exercise performance and muscle function recovery [5].

In this systematically review, we summarize the results of the studies assessing the effect of dairy products on exercise performance and on the recovery of muscle function in humans.


This systematic review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) statement [21] and was registered through the International Prospective Register of Systematic Reviews (PROSPERO registration number: CRD42018094800).

Search strategy

A literature search was conducted in the MEDLINE (via PubMed) and Web of Science (WOS) databases from their inception to 15th April 2018. The search terms as well as the search strategy and equations can be seen in detail in the Additional file 1: Table S1. Briefly, we used “dairy products”, “exercise”, “training”, “athletic performance”, “muscle strength”, “muscle fatigue”, and “muscle recovery” among others terms (see Additional file 1: Table S1) joined with Boolean operators. The reference lists of the retrieved systematic reviews and meta-analyses were reviewed to identify additional studies.

Selection criteria

The inclusion criteria used were 1) dairy product and exercise intervention (either chronic or acute) studies. The difference between the intervention and the control group/period should be in the dairy product consumption. Dairy product includes raw and processed or manufactured milk and milk-derived products. Dairy products normally come from cow but could be also from goats, sheep, reindeer, and water buffalo as defined by the National Library of Medicine (PubMed) [22]; 2) conducted in healthy humans, regardless of age or fitness level; and 3) studies including measurements of exercise performance or recovery of muscle function. We included studies that measured exercise performance quantified by fitness parameters such as maximum repetition test and isokinetic dynamometry variables [23, 24]. Moreover, we included studies that assessed muscle recovery function by subjectively measurements [e.g., ratio of perceived exertion and visual analogue scales (VAS)] or objectively measured by the use of blood markers [(e.g., creatine kinase (CK) and myoglobin)] [25]. If the same data/study was used in different original articles for different purposes, only the report that provided more detailed information about the topic of this systematic review was included.

The exclusion criteria used were 1) studies written in languages other than English or Spanish; 2) studies in which any type of protein, flavoring or sweetener was added to the consumed dairy product; moreover, colostrum (e.g., bovine colostrum), chocolate milk and breast milk were excluded from this systematic review; 3) studies in which there was no a control group.

Data extraction

The following data were collected from each included study: 1) study characteristics (author identification and reference); 2) number of participants and sex; 3) age of the participants; 4) fitness level of the participants; 5) design; 6) groups; 7) exercise intervention; 8) dairy product ingestion (e.g. cow’s milk ingestion) and placebo ingestion; 9) study outcomes; 10) results; and 11) risk of bias score.

Regarding the exercise intervention, those studies including exercises such as sprints series, isokinetic (combining eccentric and concentric contractions) or resistance (e.g. bench press) exercise or training were classified in resistance or high-intensity exercise. Those studies including exercises such as continuous cycling or cycling at different intensities (e.g. 70% peak oxygen uptake) were classified in endurance exercise.

Study quality and risk of bias assessment

The Cochrane risk of bias tool [26] was used to evaluate the risk of bias in each study. This tool assesses random sequence generations and allocation concealment, performance bias (blinding of participants and personnel), detection bias (blinding of the outcome assessment), attrition bias (incomplete outcome data), reporting bias (selective reporting), and other sources of bias.

The literature search and data extraction as well as the quality assessment were independently performed by four reviewers (JMAA, GSD, BMT and JRR), and inconsistencies were solved by consensus.


Overall results

Figure 1 presents the PRISMA consort diagram for the search strategy. The initial search retrieved 7708 articles, and a total of 11 studies were finally included after applying the inclusion and exclusion criteria (Table 1). All the included studies used cow’s milk products. A total of 7 studies (63.6%) investigated the acute effect of cow’s milk after resistance or high-intensity exercise [27,28,29,30,31,32,33], whereas three studies (27.3%) determined the acute effect of cow’s milk after endurance exercise [34,35,36]. In addition, one study [37] analyzed the long-term effect (over 12 weeks of resistance training) of exercise and cow’s milk on the maximum repetition strength of squat and bench press.

Fig. 1
figure 1

The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) flow diagram shows the identification, screening, eligibility, and inclusion of articles in the systematic review. WOS: Web of Science

Table 1 Overview of milk and dairy product studies on exercise performance and recovery of muscle function

Risk of bias within studies

The quality of the included studies was predominantly suboptimal. The methodological quality assessment is shown in Fig. 2. Details of randomization [27,28,29,30,31,32,33,34,35,36,37], allocation concealment [27,28,29,30,31,32,33,34,35,36,37], and study blinding [27,28,29,30,31,32, 35,36,37] were inadequately reported or rated as an “unclear risk” (categorized when the information was not specified in the article) for most studies.

Fig. 2
figure 2

Methodological quality of the included studies. The methodological quality was assessed using the Cochrane risk of bias tool [26]

Effects of cow’s milk on performance and muscle function recovery after resistance or high-intensity exercise

The exercise performance outcomes measured were muscular strength related variables [27,28,29,30,31,32,33, 37]. Regarding muscle function recovery, most of the studies [27,28,29,30,31,32,33] determined muscle soreness or damage using subjective scales such as VAS, and the most of them added also measures of blood biomarkers (e.g., CK or myoglobin) [27,28,29,30,31,32].

Cow’s milk attenuated losses in peak torque (maximal effort concentric knee flexion) [27, 28, 30, 31], total work of the set (6 concentric knee flexion repetitions) using isokinetic dynamometry [31], countermovement jump [27, 28, 33], rate of force development of an isometric contraction of the dominant leg quadriceps [27, 28], and sprint tests [27, 29, 32]. On the other hand, other studies observed no effect of cow’s milk on sprint recovery [28, 33], countermovement jump [32], reactive strength index [32], and bench and squat maximum strength after an exercise intervention [37].

Cow’s milk did not modify the pre-post resistance exercise changes in serum CK [27, 28, 32], myoglobin [32], high-sensitivity C-reactive protein [27, 28] and protein carbonyls [27]. In contrast, a positive effect, i.e. lower increase in CK and myoglobin concentrations, was observed from the baseline to 48 h after resistance exercise in the cow’s milk group compared with the values of the placebo beverage group [31]. Cockburn et al. [30] also showed that the increase in CK can be blunted after resistance exercise with less cow’s milk ingestion (500 mL of cow’s milk instead of 1000 mL of cow’s milk). These lower increases of CK were observed from 24 to 72 h after exercise-induced muscle damage in the hamstring and cow’s milk ingestion [29]. Cow’s milk also attenuated the skeletal troponin I increase after exercise compared with a placebo group (energy-matched carbohydrate solution) [29].

Cow’s milk did not improve muscle soreness after resistance exercise in other studies [31,32,33], whereas it had a positive effect on muscle soreness and tiredness at 72 h post resistance exercise in other [27]. Moreover, cow’s milk reduced passive soreness in males and females, as well as active muscle soreness (all from baseline to 72 h) in both sexes [29]. Similar results were found in another study that compared cow’s milk vs. energy-matched carbohydrate solution as a control at 72 h [28]. Finally, passive measurements of muscle soreness (using VAS) showed a benefit of limiting increases in muscle soreness in the group receiving less bolus cow’s milk (500 mL) compared with the high-bolus cow’s milk group (1000 mL) between the baseline and 48 h after exercise and cow’s milk ingestion [30]. Due to lack of homogeneity in the measurement of exercise performance, and on the recovery of muscle function outcomes after resistance or high-intensity exercise intervention doing a meta-analysis was not possible (see Table 1).

Effects of cow’s milk on exercise performance and muscle function recovery after endurance exercise

Cow’s milk before (2 h prior the exercise) endurance exercise improved performance in a 20-km time trial (P < 0.05) [34]. Moreover, no differences in heart rate were observed between the cow’s milk group and the placebo group [34]. In another study [35], the mean cycling time to exhaustion was the same on the placebo group trial (39.6 ± 7.3 min) compared with the cow’s milk group (39.7 ± 8.1 min; P = 0.879). Furthermore, no differences in oxygen consumption during exercise were found [35]. Finally, there was no effect of cow’s milk on changes in the rate of perceived exertion after exercise (P = 0.744) compared with the placebo group [35].

Lee et al. [36] did not observe differences in the time to volitional exhaustion independently of the beverage ingested (median, range: 103.3, 85.7–228.5 vs. 93.3, 82.4–192.3 min for the cow’s milk vs. placebo group, respectively) [36]. Cow’s milk did not alter the heart rate reached in the volitional exhaustion point nor the RPE sensations during the exercise [36]. Due to heterogeneity in the measurement of exercise performance and on the recovery of muscle function outcomes after endurance exercise doing a meta-analysis was not possible (see Table 1).


We systematically reviewed and summarized the results of the studies investigating the effects of dairy products on exercise performance or on the recovery of muscle function in humans. All the studies meeting the criteria were conducted using cow’s milk. The studies investigating the effect of cow’s milk after high-intensity exercise [27,28,29,30,31,32,33] or resistance training [37] reported contradictory results. Whereas some studies found significant effects of cow’s milk intake on performance or recovery of muscle function such as attenuated losses in peak torque, countermovement jump, rate of force development, sprint series and inflammation and markers of muscle damage, others did not find any effect. Therefore, there is currently not enough evidence to conclude whether cow’s milk has a positive effect on exercise performance and recovery of muscle function, and further studies are needed to make more definitive conclusions. The results regarding endurance exercise are similar to those observed in resistance exercise. Whereas one study observed significant effect of cow’s milk intake on performance [34] others [35, 36] did not observe any enhancing effect. The observed contradictory findings can be explained by the heterogeneity of cow’s milk ingestion, such as the amount of cow’s milk, the timing of the cow’s milk intake, and the type of intervention, as well as by the large heterogeneity of outcomes measured. Moreover, the study participants’ fitness level may also play an important role when studies are compared. In addition, doing a meta-analysis was not appropriate due to the heterogeneity between studies, which was mainly identified in the reporting of exercise performance and on the recovery of muscle function.

It has been shown that 20 g of protein might be sufficient to stimulate muscle protein synthesis after resistance exercise [38], so perhaps a greater consumption of protein (via cow’s milk) would have resulted in more positive effects. It is noteworthy, however, that one study showed similar effects after consumption of 500 and 1000 mL of cow’s milk [30]. Volek et al. [37] found no differences between the effects of cow’s milk vs. juice (both groups consumed 708 mL daily) after a resistance exercise intervention in maximum repetition strength (12 weeks of resistance training). Both Lee et al. [36] and Watson et al. [35] found no effect of cow’s milk on time to volitional exhaustion, heart rate, expired gases and RPE after endurance exercise. In contrast, Upshaw et al. [34] found that low-fat cow’s milk (2262 ± 299 mL) improved the time in a 20-km time trial test after glycogen-lowering exercise compared with that of a placebo group (2262 ± 290 mL low-energy drink). Regarding the intensity of the exercise, one study [35] reported that the heart rate during endurance exercise in a cow’s milk trial test (2263 ± 241 mL) was higher than that during a carbohydrate trial test (2280 ± 249 mL), yet no differences in oxygen consumption during exercise were observed. The intensity and exercise performed in both studies was similar [35, 36], while in the study by Upshaw et al. [34], a glycogen-lowering exercise was performed before both cow’s milk ingestion and the examined exercise. The amount of cow’s milk ingested in the study by Upshaw et al. [34] and in the study by Watson et al. [35] was similar, and therefore, the differences in recovery between the groups might be partially explained by differences in the fitness levels of the participants [35, 36]. In the study by Upshaw et al. [34], the participants were trained cyclists, while in the other study, the participants were regularly active individuals.

Philips et al. [39] in their review focused on the evidence showing the differences in responses of muscle protein synthesis and muscle protein accretion in humans, concluded that cow’s milk-based proteins (whey and casein) appear to be better than carbohydrate beverages in the promotion of hypertrophy. Moreover, they highlighted the importance of the dose-response in the studies since the difference in leucine content (as is present in cow’s milk) may have an important influence in maintaining and possibly increasing the muscle mass [39]. Furthermore, leucine could have an impact in the recovery process (e.g. in the muscle protein synthesis and muscle protein accretion). Maybe the contradictory results obtained in exercise performance in our systematic review could be partially explained by the different amount of cow’s milk (and therefore leucine content) provided in the selected studies.

It is assumed that a single cow’s milk bolus intake increases net amino acid synthesis in young healthy sedentary volunteers [40]. Moreover, the consumption of protein and carbohydrates together, as is presented in cow’s milk, results in a higher rate of protein synthesis compared to that from the intake of these nutrients separately [41, 42]. Regarding protein consumption, sufficient protein intake is necessary to stimulate protein synthesis as we mentioned previously [38]. Therefore, it is biologically plausible that cow’s milk consumption after a bout of exercise can stimulate protein synthesis metabolism [6, 7]. Muscle membrane damage following exercise occurs as a result of mechanical stress during the first phase of muscular damage, with further disruption via the lysosomal pathway during the next phase [43]. The lack of a positive effect of cow’s milk on some blood markers (e.g., protein carbonyls and high-sensitivity C-reactive protein) [27, 28, 32] may suggest that the ingestion of cow’s milk could affect other metabolic pathways. For example, the CK concentration increases after exercise, but no clear CK blunting effects were observed from cow’s milk, while the skeletal troponin I rise was blunted in the cow’s milk group [29]. Controversially, in another study it was shown that the CK increase can be attenuated with lower cow’s milk intake (500 mL instead of 1000 mL) [30], and an effect on myoglobin was also observed between the baseline and 48 h after exercise [31] from cow’s milk ingestion. Of note is that in both of these studies, participants played regularly in team sports, and this fact could result in less muscle damage due their fitness level. In their review, Sousa et al. [44] recommend the ingestion of 0.8–1.2 g carbohydrate/kg/h and 0.2–0.4 g protein/kg/h preferably after the exercise, with a minimum of 20 g high-quality protein for improve the recovery after exercise. However, some controversial still exist regarding the correct timing and if carbohydrate and protein have to be consumed right after the exercise [44, 45]. Following these recommendations by Sousa et al. [44], maybe the negative results found in some of the included studies could be partially explained for either an insufficient amount of cow’s milk (i.e. not enough protein and/or leucine) or the timing of ingestion. Regarding the time of ingestion, most of the studies provided the beverage (e.g. cow’s milk, placebo, etc.) immediately after the exercise [27,28,29,30,31,32], while others offered the beverage during the exercise [35, 36], and one [34] offered the beverage immediately after the exercise and every 30 min. In Volek et al. [37] the beverage consumption was daily.

Regarding muscle soreness perception, whereas several studies did not find a positive effect of cow’s milk [31,32,33, 36], others observed significant differences in both active and passive muscle soreness between cow’s milk and control groups (~ 500 mL) [27,28,29,30]. However, VAS muscle soreness perception is a more subjective outcome than blood markers, and it is more difficult to establish whether these differences could be explained by the treatment (e.g., cow’s milk vs. placebo), by the physical condition of participants (e.g., team sport players vs. individuals not habituated to exercise), or by unaccustomed sensations after resistance (i.e., repeated eccentrics contractions).

The current review has several limitations. First, there is a high degree of heterogeneity among the analyzed studies, in part due to differences in the type, intensity, volume, frequency, and duration of the interventions, as well as in the outcome measures, and, for these reasons a meta-analysis was not possible. This review is also limited by the suboptimal methodological quality of the included interventions. Finally, because the search was limited to articles published in English or Spanish and gray literature was not consulted, the language restrictions and unpublished studies might slightly modify our results. Therefore, the results should be taken with caution, and more research on the effects of cow’s milk and dairy products is required before definitive recommendations can be provided.


In conclusion, based on the current evidence, it cannot be determined whether cow’s milk has a positive effect on exercise performance and recovery of muscle function in humans, due to the limited number of studies included in this systematic review. Nevertheless, since cow’s milk is a source of protein, carbohydrates, calcium and other nutrients, and thus may lead to an increase in the serum amino acid concentration and, therefore, helping the muscle damage repair process. In line with this, some studies included found significant effects of cow’s milk intake on performance and recovery of muscle function. For these reasons, more and better study designs such as blinding the beverage to both, participants and personnel, generate a random sequence of beverage group, etc. are needed to demonstrate its usefulness as a sport nutrition-related supplement.



Creatine kinase


Countermovement jump


Heart rate


High-sensitivity C-reactive protein






Medical subject heading


Maximum voluntary isometric contractions


Protein carbonyls


Radians per second


Rate of force development


Maximum repetition


Ratio of perceived exertion


Revolutions per minute


Reactive strength index


Skeletal troponin I


Visual analogue scales

VO2 :

Volume of oxygen consumption

VO2peak :

Peak oxygen uptake


Web of Science


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This study is part of a Ph.D. thesis conducted in the Biomedicine Doctoral Studies of the University of Granada, Spain.


The study was supported by the Spanish Ministry of Education (FPU 13/04365 and 15/04059), by the Redes temáticas de investigación cooperativa RETIC (Red SAMID RD16/0022), and by the University of Granada Plan Propio de Investigación 2016 -Excellence actions: Unit of Excellence on Exercise and Health (UCEES) - and Plan Propio de Investigación 2018 - Programa Contratos-Puente, and the Junta de Andalucía, Consejería de Conocimiento, Investigación y Universidades (ERDF: ref. SOMM17/6107/UGR), and by the Interprofessional Dairy Organization (INLAC) of Spain. The sponsor had no role in the design of the studies included in the supplement; in the collection, analyses, or interpretation of the data; in the writing of the manuscript; or in the decision to publish the results.

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JMAA, GS-D, BM-T, and JRR contributed to the design of the systematic review; JMAA, GS-D, BM-T, IL and JRR contributed to the analysis and interpretation of data; JMAA and JRR drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Juan M. A. Alcantara.

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Additional file

Additional file 1:

Table S1. Search equations for both databases. (DOCX 14 kb)

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Alcantara, J.M.A., Sanchez-Delgado, G., Martinez-Tellez, B. et al. Impact of cow’s milk intake on exercise performance and recovery of muscle function: a systematic review. J Int Soc Sports Nutr 16, 22 (2019).

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