Amino acids are theorized to enhance performance in a variety of ways, such as increasing the secretion of anabolic hormones, modifying fuel use during exercise, preventing adverse effects of overtraining, and preventing mental fatigue. The following discussion highlights research regarding the ergogenic effects of individual amino acids, various combinations of amino acids, and several special protein dietary supplements.
Tryptophan (TRYP), is a precursor for serotonin, a brain neurotransmitter theorized to suppress pain. Free tryptophan (fTRYP) enters the brain cells to form serotonin. Thus, tryptophan supplementation has been used to increase serotonin production in attempts to increase tolerance to pain during intense exercise. One study reported significant improvements in time to exhaustion at 80 percent of maximal oxygen uptake, accompanied by significant reductions in the rating of perceived exertion . However, research with a more appropriate experimental design did not replicate these findings . Moreover, other investigators reported no effect of TRYP supplementation on aerobic endurance performance at 70–75 percent of maximal oxygen uptake . Tryptophan does not appear to be an effective ergogenic .
Branched chain amino acids (BCAA)
Some investigators believe that increased levels of serotonin may cause fatigue . During prolonged aerobic endurance exercise, muscle glycogen may become depleted and the muscle may increase its reliance on BCAA for fuel, decreasing the plasma BCAA:fTRYP ratio. Because BCAA compete with fTRYP for entry into the brain, a low BCAA:fTRYP ratio would facilitate the entry of fTRYP to the brain and the formation of serotonin. Hypothetically, BCAA supplementation may delay central nervous system fatigue and enhance performance in prolonged aerobic endurance events by increasing the BCAA:fTRYP ratio and mitigating the formation of serotonin.
BCAA supplementation has been studied for its effects on various types of exercise performance, including ratings of perceived exertion (RPE) during exercise and mental performance following exercise. In general, the findings are equivocal, as are the conclusions from several recent reviews. One investigator concluded that BCAA supplementation reduces RPE and mental fatigue during prolonged exercise and improves cognitive performance after exercise, and also suggests that in some situations BCAA supplementation may improve physical performance, such as during exercise in the heat or in actual competitive races where central fatigue may be more pronounced than in laboratory experiments . However, other reviewers conclude that most studies show no effects of BCAA supplementation on performance, such as prevention of fatigue during prolonged exercise [13, 14] two recent studies support these conclusions. Watson and others  reported no beneficial effects of BCAA supplementation, consumed before and during prolonged cycling to exhaustion at 50 percent VO2max in the heat, on performance time, heart rate, and core or skin temperature. Cheuvront and others  reported similar findings with subjects exercising in the heat, noting no significant effect of BCAA supplementation on time-trial performance, cognitive performance, mood, perceived exertion, or perceived thermal comfort. Although current research does not support an ergogenic effect of BCAA supplementation, most investigators recommend additional research.
Glutamine may be theorized to be ergogenic in various ways . It is an important fuel for some cells of the immune system, such as lymphocytes and macrophages, which may be decreased with prolonged intense exercise, such as that related to overtraining. Glutamine may also promote muscle glycogen synthesis, and has been studied for potential enhancement of muscular strength.
Several investigators theorize that athletes who overtrain may experience decreased plasma glutamine levels, which may impair functions of the immune system and predispose the athlete to various illnesses [17, 18]. Illness may impair training and eventual performance. Research findings are equivocal, with some studies reporting lower incidence rates of infection among athletes who consumed a glutamine-supplement drink following intense training . However, others reported that although glutamine supplementation helped maintain plasma glutamine levels following intense exercise, it had no effect on various tests of the immune response . Recent reviews indicated that there is little support from controlled studies to recommend glutamine ingestion for enhanced immune function [14, 21].
Although glutamine may simulate muscle glycogen synthesis, reviewers recently concluded that there is no advantage over ingestion of adequate carbohydrate alone . Moreover, several recent studies indicate that neither short-term nor long-term glutamine supplementation has an ergogenic effect on muscle mass or strength performance. Glutamine supplementation one hour prior to testing had no effect on resistance exercise to fatigue, nor did six weeks of glutamine supplementation during resistance training increase lean muscle mass or strength more so than the placebo treatment [22, 23].
Potassium and magnesium aspartates are salts of aspartic acid, an amino acid. They have been used as ergogenics, possibly by enhancing fatty acid metabolism and sparing muscle glycogen utilization or by mitigating the accumulation of ammonia during exercise. The effect of aspartate supplementation on physical performance is equivocal, but about 50 percent of the available studies have indicated enhanced performance in exercise tests of aerobic endurance . Additional research is needed to study the potential ergogenicity and underlying mechanisms of aspartate salt supplementation.
Arginine supplementation may be theorized to be ergogenic because it is a substrate for nitric oxide (NO) synthesis, a potent endogenous vasodilator that may benefit blood flow and endurance capacity. Several studies involving patients with peripheral arterial disease or clinical symptoms of stable angina pectoris have shown improved exercise capacity with arginine supplementation [24, 25]. However, research involving the independent effect of arginine supplementation on the aerobic endurance capacity of healthy athletes has not been uncovered .
Ornithine, lysine and arginine
Ornithine, lysine and arginine have been used in attempts to increase human growth hormone (HGH) production, the theory being to increase lean muscle mass and strength. However, although limited data are available, a number of well-controlled studies, several with experienced weight lifters, reported no increases in HGH levels or various measures of muscular strength or power [26–28].
Chromiak and Antonio  reviewed the scientific studies on growth-hormone releasing amino acids (ornithine, lysine and arginine) and indicated that oral doses that are great enough to induce significant growth hormone release are likely to cause gastrointestinal discomfort. Moreover, they reported that no studies found that pre-exercise oral amino acid supplementation augments growth hormone release. They also concluded that no appropriately conducted scientific studies found that oral supplementation with such amino acids before strength training increases muscle mass and strength to a greater extent than strength training alone. They do not recommend the use of specific amino acids to stimulate growth hormone release.
Tyrosine is a precursor for the catecholamine hormones and neurotransmitters, specifically epinephrine, norepinephrine, and dopamine. Some have suggested that inadequate production of these hormones or transmitters could compromise optimal physical performance. Thus, as a precursor for the formation of these hormones and neurotransmitters, tyrosine has been suggested to be ergogenic. However, in a well-designed placebo-controlled, crossover study, Sutton  and others found that tyrosine supplementation (150 milligrams/kilogram body weight) consumed 30 minutes prior to taking a series of physical performance tests significantly increased plasma tyrosine levels, but had no significant ergogenic effects on aerobic endurance, anaerobic power, or muscle strength.
Taurine is a non-essential sulfur-containing amino acid, but it lacks a genetic codon to be incorporated into proteins or enzymes. Nevertheless, it plays a role in several metabolic processes, such as heart contraction and antioxidant activity. Taurine is an ingredient in several so-called energy drinks, such as Red Bull.
Baum and Weiss  reported that Red Bull, which contains taurine and caffeine, as compared to a similar drink without taurine, favorably influence cardiac parameters, mainly an increased stroke volume, during recovery after exercise; however, physical performance was not tested. However, Zhang and others  reported that 7 days of taurine supplementation induced significant increases in VO2max and cycle ergometer exercise time to exhaustion; the ergogenic effects were attributed to taurine's antioxidant activity and protection of cellular properties.
Amino Acid Cocktails
Providing an ample supply of essential amino acids to the muscle within 1–3 hours before or following exercise may help to further muscle protein synthesis. Gibala  indicated that consumption of a drink containing about 0.1 gram of essential amino acids per kilogram of body weight (7 grams for a 70-kilogram athlete) during the first few hours of recovery from heavy resistance exercise will produce a transient, net positive increase in muscle protein balance. Gibala also noted that it is uncertain if ingesting amino acids, either alone or combined with carbohydrate, immediately before exercise or during recovery further enhances the rate of muscle protein buildup during recovery. Some investigators have suggested that it may be helpful to consume, throughout the day, multiple small meals having adequate protein. Gibala indicates that although these strategies will promote a net "anabolic" environment in the body, it remains to be determined if the acute effects of supplementation eventually lead to greater gains in muscle mass following habitual training. Others also note that small amounts of amino acids, combined with carbohydrates, can transiently increase muscle protein anabolism, but it has yet to be determined if these transient responses result in an appreciable increase in muscle mass over a prolonged training period [42, 34].
Overall, given these findings, consuming a small amount of protein and carbohydrate, either as a protein/carbohydrate energy drink or whole foods, before or after exercise training may be prudent behavior for many athletes.