Effects of 8 weeks of Xpand® 2X pre workout supplementation on skeletal muscle hypertrophy, lean body mass, and strength in resistance trained males
© Lowery et al.; licensee BioMed Central Ltd. 2013
Received: 19 June 2013
Accepted: 2 October 2013
Published: 9 October 2013
Xpand® 2X is a proprietary blend comprised of branched chain amino acids, creatine monohydrate, beta-alanine (CarnoSyn®), quercetin, coenzymated B-vitamins, alanyl-glutamine (Sustamine®), and natural nitrate sources from pomegranate and beet root extracts purported to enhance the neuromuscular adaptations of resistance training. However to date, no long-term studies have been conducted with this supplement. The purpose of this study was to investigate the effects of a multi-ingredient performance supplement (MIPS) on skeletal muscle hypertrophy, lean body mass and lower body strength in resistance-trained males.
Twenty resistance-trained males (21.3 ± 1.9 years) were randomly assigned to consume a MIPS or a placebo of equal weight and volume (food-grade orange flavors and sweeteners) in a double-blind manner, 30 minutes prior to exercise. All subjects participated in an 8-week, 3-day per week, periodized, resistance-training program that was split-focused on multi-joint movements such as leg press, bench press, and bent-over rows. Ultrasonography measured muscle thickness of the quadriceps, dual-energy X-ray absorptiometry (DEXA) determined lean body mass, and strength of the bench press and leg press were determined at weeks 0, 4, and 8 of the study. Data were analyzed with a 2 × 3 repeated measures ANOVA with LSD post hoc tests utilized to locate differences.
There was a significant group-by-time interaction in which the MIPS supplementation resulted in a significant (p < 0.01) increase in strength of the bench press (18.4% vs. 9.6%) compared with placebo after 4 and 8 weeks of training. There were no significant group by time interactions between MIPS supplementation nor the placebo in leg press strength (p = .08). MIPS supplementation also resulted in a significant increase in lean body mass (7.8% vs. 3.6%) and quadriceps muscle thickness (11.8% vs. 4.5%) compared with placebo (group*time, p <0.01).
These results suggest that this MIPS can positively augment adaptations in strength, and skeletal muscle hypertrophy in resistance-trained men.
KeywordsPre-workout Performance Hypertrophy Supplementation Sports nutrition
Sports nutrition scientists have attempted to augment training induced adaptations through a number of supplementation protocols, which generally attempt to enhance and/or speed skeletal muscle development. Research-supported interventions include the provision of the branched chain amino acids (BCAAs), creatine monohydrate, and several other amino acids and their byproducts. Additionally, it has been postulated that a critical period for supplementation is prior (e.g., 30–60 minutes before) to the workout. However, little research have been done on the effects of multi-ingredient pre-workout supplementation on long-term changes in skeletal muscle hypertrophy, lean body mass, and performance.
Multi-ingredient performance supplements (MIPS) may be comprised of ingredients such as BCAAs, creatine, beta-alanine, agmatine sulfate, taurine, creatinol-o-phosphate, and a combination of nitrate-containing compounds purported to enhance both acute performance and long-term neuromuscular adaptations[3–5]. Each of these ingredients has been studied individually for their positive effects on human performance adaptations. For example, pure creatine monohydrate, beta-alanine, and creatinol-o-phosphate have each been shown to enhance muscle adaptations[6, 7]. In addition to supporting skeletal muscle hypertrophy, a vast array of nootropics, which have previously been demonstrated to augment preworkout performance including the combinations of glucuronolactone, tyrosine, caffeine, and rhodiola rosea are found within the MIPS[8–10]. Lastly, the MIPS contains agmatine sulfate, pomegranate and beet root extracts, which has been shown to enhance skeletal muscle blood flow. However, information is lacking as to how resistance-training individuals will respond to the combination of these ingredients.
Therefore, the primary purpose of this study was to investigate the effects of 8 weeks of a MIPS in resistance-trained individuals during a periodized resistance training program on skeletal muscle hypertrophy, lean body mass, and strength relative to a placebo matched control.
Our study consisted of a randomized, double-blind, diet-controlled design, wherein a pre-workout supplemented and a placebo supplemented group were assigned to an 8-week periodized resistance training protocol. Muscle thickness, lean body mass, and strength were examined collectively at the end of weeks 0, 4, and 8 to assess the effects of the MIPS.
Twenty-four resistance-trained males aged 21.3 ± 1.9 years with a respective average leg press and bench press of 3.40 ± 0.2 and 1.38 ± 0.9 times their bodyweight and a minimum of 1 year of resistance training experience were recruited for the study. Subjects could not participate if they were currently taking any medications including anti-inflammatory agents, any performance enhancing supplements, or if they smoked. Specifically, subjects could not have taken nutritional supplements for at least three months prior to data collection. Inclusion criteria included that subjects had previously been performing a minimum of 3 days per week resistance training. Each participant signed an informed consent approved by the University of Tampa Institutional Review Board before participating in the study.
Resistance training protocol
Resistance training protocol
hyper - leg&chest
hyper - back&delts
90° Bent Rows
Close Grip Bench Press
Dumbbell incline Bench Press
Close Grip Bench Press
Tricep cable extension
Rep Scheme: 6 - 15
Rep Scheme: 6 - 15
Rep Scheme: 1 - 5
Strength, lean body mass, and muscle mass testing
Strength was assessed via 1-RM testing of the leg press and bench press. Lean Body Mass was determined on a Lunar Prodigy dual energy x-ray absorptiometry (DEXA) apparatus (software version, enCORE 2008, Madison, Wisconsin, U.S.A.). Skeletal muscle hypertrophy was determined via changes in quadriceps ultrasonography (determined by taking the combined muscle thickness of the vastus lateralis (VL) and vastus intermedius (VI) muscles. The reliability of measurements of muscle thickness by the same investigator was 0.98. These tests were performed at the end of weeks 0,4, and 8 in replacement of the heavy day in order to ensure full recovery (Friday).
Supplementation and diet control
Prior to the study, subjects were randomly assigned to consume Xpand® 2X (MIPS) or an isocaloric placebo of equal weight and volume (food-grade orange flavors and sweeteners) in a double-blind manner 30 minutes prior to exercise. On the non-training days, subjects were instructed to consume three equal servings spread evenly throughout the day with breakfast, lunch, and dinner. Two weeks prior to as well as throughout the study, subjects were placed on a diet consisting of 25% protein, 50% carbohydrates, and 25% fat by a registered dietitian who specializes in sport nutrition. Post study, analysis of the subjects recorded diets revealed diets consisted of 23% protein, 46% carbohydrates, and 31% fat, with no differences between groups. Subjects met as a group with the dietitian and were given individual meal plans at the beginning of the study as well as instructed not to consume any alcohol as it can dehydrate and impair the subjects’ results. Upon completion of the study, all subjects’ diets were analyzed to ensure compliance. Since the dietician worked weekly with the subjects, no subject’s data had to be removed due to non-compliance. No adverse side effects were found in either group.
Repeated measures analysis of variance was performed to assess group, time, and group by time interactions. If any main effects were found, an LSD post hoc was employed to locate differences. An alpha priori of p < 0.05 was established. Statistica (StatSoft®, Tulsa, OK, USA) was used for all statistical analysis.
The primary purpose of this study was to investigate the effects of 8 weeks of a MIPS in resistance-trained individuals during a periodized resistance training program on skeletal muscle hypertrophy, lean body mass, and strength relative to a placebo matched control. The primary findings of this research were in congruence with our hypotheses that Xpand® 2X supplementation can improve adaptations in skeletal muscle hypertrophy, LBM, and strength.
Skeletal muscle hypertrophy and lean body mass
This MIPS contains a proprietary blend of ingredients reported previously to augment the accretion of skeletal muscle. For example, creatine monohydrate has been reported as the most effective ergogenic aid currently available regarding LBM and high-intensity exercise capacity, particularly in untrained individuals. Creatine and its various forms have been thoroughly researched, yet to date no study has shown any form of creatine to be superior to creatine monohydrate, which was used in this study (23, 33). Supplementation with creatine can increase total resistance training volume via ATP re-synthesis, and total training volume has been closely linked with skeletal muscle accretion. Additionally, creatine supplementation has been demonstrated to increase the activation of satellite cells and myonuclei in muscle following chronic resistance training. Moreover it is conceivable that the osmotic pressure created by creatine that increases the hydration status of cells, resulting in potentially hypertrophic effects. This mechanism of action is what causes creatine to increase strength, but can benefit almost every body system, including the brain, bones, muscles, and liver. Lastly, long term studies have observed that those supplementing with creatine experience 200% increases in LBM compared to placebo.
Branched chain amino acids have previously been shown as efficacious in the accretion of skeletal muscle mass. One BCAA of particular interest is leucine, which has been shown to increase muscle protein synthesis (MPS) without the presence of the other essential amino acids Additionally, Karlsson et al. found that supplementation with BCAAs during resistance exercise results in greater phosphorylation of ribosomal S6 kinase, a rate limiting enzyme in the signaling network responsible for regulation of protein synthesis in skeletal muscles. Moreover, BCAAs seem to decrease soreness after eccentric exercise and, they prevent declines in both testosterone and power following an overreaching cycle.
Beta-Alanine supplementation has consistently been demonstrated to augment muscle carnosine concentrations in humans[22–25]. Harris et al. concluded that carnosine plays an essential role as an intracellular buffer within the skeletal muscle of humans. More importantly, beta-alanine supplementation has been shown to enhance physical performance during high intensity exercise bouts while also delaying the onset of neuromuscular fatigue.
Agmatine is a derivative of the amino acid arginine. Agmatine has been studied for its impact on nitric oxide, wellbeing, and hormone status[27, 28]. Agmatine has been noted to support nitric oxide (NO) production via stimulation of endothelial nitric oxide synthase (eNOS).[29–31]. This process is essential for the proper functioning of the polyamine biosynthetic pathways to occur[29–31]. The body’s organs require polyamines for their growth, renewal, and metabolism. Polyamines also have a profound stabilizing effect on a cell DNA and are essential to the healthy function of the nervous system[29, 30]. Therefore, these pathways, although not fully elucidated, play an important role in normal cell homeostasis.
Lastly, Creatinol-O-Phosphate (COP) is known primarily for its abilities as an intracellular buffer. Creatinol-O-Phosphate has been shown to assist in stabilizing intracellular and extracellular pH levels, ultimately prolonging anaerobic glycolysis in the presence of lactic acid. Creatinol-O-Phosphate has also been shown to activate satellite cells in skeletal muscle, theoretically increasing their capacity for muscle growth. To date this is the first study that we are aware of to analyze this specific combination of ingredients.
Strength is one of the most critical attributes underlying success in sport. The collective results of the present study suggest that bench press strength following Xpand® 2X supplementation are optimized within the context of a periodized training split. Most of the individual ingredients found within the pre-workout matrix have been demonstrated to individually enhance a variety of strength measures. For example, creatine monohydrate has repeatedly been shown to augment strength. In addition to creatine, this MIPS contains a number of nootropics including glucuronolactone, caffeine, and taurine, which can possibly decrease perceived exertion and augment intra-workout performance. For example, caffeine has been demonstrated to enhance strength acutely through an adenosine receptor antagonist mediated mechanism and subsequent central nervous system stimulation.
Research suggests that taurine might increase the mechanical threshold for skeletal muscle contraction, promote intracellular membrane stabilization, and increase membrane polarization. Taurine has also been noted for its performance enhancing, antioxidant, and nerve conduction effects[22, 35]. Moreover the combination of caffeine and taurine has previously been shown to augment performance to a greater degree than caffeine alone.
The collective results of our research indicate that Xpand® 2X (MIPS) supplementation results in significant increases in bench press strength, hypertrophy, and lean body mass in resistance-trained men. Therefore, coaches, trainers, and athletes may increase the benefits of their workouts by supplementing with MIPS prior to exercise. Future research should investigate if supplementation with MIPS would result in better adaptations than supplementation with each of the ingredients alone.
- Wilson JM: Effects of amino acids and their metabolites on aerobic and anaerobic sports. Strength Condition J. 2012, 34: 33-48.View ArticleGoogle Scholar
- Cribb PJ, Hayes A: Effects of supplement timing and resistance exercise on skeletal muscle hypertrophy. Med Sci Sports Exer. 2006, 38: 1918-1925. 10.1249/01.mss.0000233790.08788.3e.View ArticleGoogle Scholar
- Ormsbee MJ, Mandler WK, Thomas DD, Ward EG, Kinsey AW, Simonavice E, Panton LB, Kim JS: The effects of six weeks of supplementation with multi-ingredient performance supplements and resistance training on anabolic hormones, body composition, strength, and power in resistance-trained men. J Int Soc Sports Nutr. 2012, 9: 49-10.1186/1550-2783-9-49.PubMed CentralView ArticlePubMedGoogle Scholar
- Shelmadine B, Cooke M, Buford T, Hudson G, Redd L, Leutholtz B, Willoughby DS: Effects of 28 days of resistance exercise and consuming a commercially available pre-workout supplement, NO-Shotgun(R), on body composition, muscle strength and mass, markers of satellite cell activation, and clinical safety markers in males. J Int Soc Sports Nutr. 2009, 6: 16-10.1186/1550-2783-6-16.PubMed CentralView ArticlePubMedGoogle Scholar
- Spillane M, Schwarz N, Leddy S, Correa T, Minter M, Longoria V, Willoughby DS: Effects of 28 days of resistance exercise while consuming commercially available pre- and post-workout supplements, NO-Shotgun(R) and NO-Synthesize(R) on body composition, muscle strength and mass, markers of protein synthesis, and clinical safety markers in males. Nutr Metab. 2011, 8: 78-10.1186/1743-7075-8-78.View ArticleGoogle Scholar
- Hoffman J, Ratamess N, Kang J, Mangine G, Faigenbaum A, Stout J: Effect of creatine and beta-alanine supplementation on performance and endocrine responses in strength/power athletes. Int J Sport Nutr Exer Metab. 2006, 16: 430-446.Google Scholar
- Nicaise J: Creatinol O-phosphate (COP) and muscular performance: a controlled clinical trial. Curr Therapeut Res Clin Exp. 1975, 17: 531-534.Google Scholar
- Neri DF, Wiegmann D, Stanny RR, Shappell SA, McCardie A, McKay DL: The effects of tyrosine on cognitive performance during extended wakefulness. Aviat Space Environ Med. 1995, 66: 313-319.PubMedGoogle Scholar
- Costill DL, Dalsky GP, Fink WJ: Effects of caffeine ingestion on metabolism and exercise performance. Med Sci Sports. 1978, 10: 155-158.PubMedGoogle Scholar
- De Bock K, Eijnde BO, Ramaekers M, Hespel P: Acute Rhodiola rosea intake can improve endurance exercise performance. Int J Sport Nutr Exer Metab. 2004, 14: 298-307.Google Scholar
- de Nigris F, Williams-Ignarro S, Sica V, Lerman LO, D’Armiento FP, Byrns RE, Casamassimi A, Carpentiero D, Schiano C, Sumi D: Effects of a pomegranate fruit extract rich in punicalagin on oxidation-sensitive genes and eNOS activity at sites of perturbed shear stress and atherogenesis. Cardiovasc Res. 2007, 73: 414-423. 10.1016/j.cardiores.2006.08.021.View ArticlePubMedGoogle Scholar
- Kraemer WJ, Spiering BA, Volek JS, Martin GJ, Howard RL, Ratamess NA, Hatfield DL, Vingren JL, Ho JY, Fragala MS: Recovery from a national collegiate athletic association division I football game: muscle damage and hormonal status. J Strength Cond Res. 2009, 23: 2-10. 10.1519/JSC.0b013e31819306f2.View ArticlePubMedGoogle Scholar
- Monteiro AG, Aoki MS, Evangelista AL, Alveno DA, Monteiro GA, Picarro Ida C, Ugrinowitsch C: Nonlinear periodization maximizes strength gains in split resistance training routines. J Strength Cond Res. 2009, 23: 1321-1326. 10.1519/JSC.0b013e3181a00f96.View ArticlePubMedGoogle Scholar
- Buford TW, Kreider RB, Stout JR, Greenwood M, Campbell B, Spano M, Ziegenfuss T, Lopez H, Landis J, Antonio J: International Society of Sports Nutrition position stand: creatine supplementation and exercise. J Int Soc Sports Nutr. 2007, 4: 6-10.1186/1550-2783-4-6.PubMed CentralView ArticlePubMedGoogle Scholar
- Dangott B, Schultz E, Mozdziak PE: Dietary creatine monohydrate supplementation increases satellite cell mitotic activity during compensatory hypertrophy. Int J Sports Med. 2000, 21: 13-16. 10.1055/s-2000-8848.View ArticlePubMedGoogle Scholar
- Parise G, Mihic S, MacLennan D, Yarasheski KE, Tarnopolsky MA: Effects of acute creatine monohydrate supplementation on leucine kinetics and mixed-muscle protein synthesis. J Appl Physiol. 2001, 91: 1041-1047.PubMedGoogle Scholar
- Kraemer WJ, Volek JS: Creatine supplementation. Its role in human performance. Clin Sports Med. 1999, 18: 651-666. 10.1016/S0278-5919(05)70174-5. ixView ArticlePubMedGoogle Scholar
- Norton LE, Layman DK: Leucine regulates translation initiation of protein synthesis in skeletal muscle after exercise. J Nutr. 2006, 136: 533S-537S.PubMedGoogle Scholar
- Karlsson HK, Nilsson PA, Nilsson J, Chibalin AV, Zierath JR, Blomstrand E: Branched-chain amino acids increase p70S6k phosphorylation in human skeletal muscle after resistance exercise. Am J Physiol Endocrinol Metab. 2004, 287: E1-E7. 10.1152/ajpendo.00430.2003.View ArticlePubMedGoogle Scholar
- Jackman SR, Witard OC, Jeukendrup AE, Tipton KD: Branched-chain amino acid ingestion can ameliorate soreness from eccentric exercise. Med Sci Sports Exerc. 2010, 42: 962-970. 10.1249/MSS.0b013e3181c1b798.View ArticlePubMedGoogle Scholar
- Kraemer WJ, Ratamess NA, Volek JS, Hakkinen K, Rubin MR, French DN, Gomez AL, McGuigan MR, Scheett TP, Newton RU: The effects of amino acid supplementation on hormonal responses to resistance training overreaching. Metab: Clin Exper. 2006, 55: 282-291. 10.1016/j.metabol.2005.08.023.View ArticleGoogle Scholar
- Harris RC, Tallon MJ, Dunnett M, Boobis L, Coakley J, Kim HJ, Fallowfield JL, Hill CA, Sale C, Wise JA: The absorption of orally supplied beta-alanine and its effect on muscle carnosine synthesis in human vastus lateralis. Amino acids. 2006, 30: 279-289. 10.1007/s00726-006-0299-9.View ArticlePubMedGoogle Scholar
- Hill CA, Harris RC, Kim HJ, Harris BD, Sale C, Boobis LH, Kim CK, Wise JA: Influence of beta-alanine supplementation on skeletal muscle carnosine concentrations and high intensity cycling capacity. Amino acids. 2007, 32: 225-233. 10.1007/s00726-006-0364-4.View ArticlePubMedGoogle Scholar
- Derave W, Ozdemir MS, Harris RC, Pottier A, Reyngoudt H, Koppo K, Wise JA, Achten E: beta-Alanine supplementation augments muscle carnosine content and attenuates fatigue during repeated isokinetic contraction bouts in trained sprinters. J Appl Physiol. 2007, 103: 1736-1743. 10.1152/japplphysiol.00397.2007.View ArticlePubMedGoogle Scholar
- Baguet A, Koppo K, Pottier A, Derave W: Beta-alanine supplementation reduces acidosis but not oxygen uptake response during high-intensity cycling exercise. Eur J Appl Physiol. 2010, 108: 495-503. 10.1007/s00421-009-1225-0.View ArticlePubMedGoogle Scholar
- Artioli GG, Gualano B, Smith A, Stout J, Lancha AH: Role of beta-alanine supplementation on muscle carnosine and exercise performance. Med Sci Sports Exer. 2010, 42: 1162-1173.View ArticleGoogle Scholar
- Horyn O, Luhovyy B, Lazarow A, Daikhin Y, Nissim I, Yudkoff M: Biosynthesis of agmatine in isolated mitochondria and perfused rat liver: studies with 15N-labelled arginine. Biochem J. 2005, 388: 419-425. 10.1042/BJ20041260.PubMed CentralView ArticlePubMedGoogle Scholar
- Molderings GJ, Haenisch B: Agmatine (decarboxylated L-arginine): physiological role and therapeutic potential. Pharmacol Therapeut. 2012, 133: 351-365. 10.1016/j.pharmthera.2011.12.005.View ArticleGoogle Scholar
- Abe K, Abe Y, Saito H: Agmatine suppresses nitric oxide production in microglia. Brain Res. 2000, 872: 141-148. 10.1016/S0006-8993(00)02517-8.View ArticlePubMedGoogle Scholar
- Gao Y, Gumusel B, Koves G, Prasad A, Hao Q, Hyman A, Lippton H: Agmatine: a novel endogenous vasodilator substance. Life Sci. 1995, 57: PL83-PL86. 10.1016/0024-3205(95)02011-7.View ArticlePubMedGoogle Scholar
- Raasch W, Regunathan S, Li G, Reis DJ: Agmatine, the bacterial amine, is widely distributed in mammalian tissues. Life Sci. 1995, 56: 2319-2330. 10.1016/0024-3205(95)00226-V.View ArticlePubMedGoogle Scholar
- Wilson JM, Duncan NM, Marin PJ, Brown LE, Loenneke JP, Wilson SM, Jo E, Lowery RP, Ugrinowitsch C: Meta-analysis of post activation Potentiation and power: effects of conditioning activity, volume, gender, rest periods, and training status. J Strength Cond Res. 2012, 27 (3): 854-View ArticleGoogle Scholar
- Becque MD, Lochmann JD, Melrose DR: Effects of oral creatine supplementation on muscular strength and body composition. Med Sci Sports Exer. 2000, 32: 654-658. 10.1097/00005768-200003000-00016.View ArticleGoogle Scholar
- Goldstein ER, Ziegenfuss T, Kalman D, Kreider R, Campbell B, Wilborn C, Taylor L, Willoughby D, Stout J, Graves BS: International society of sports nutrition position stand: caffeine and performance. J Int Soc Sports Nutr. 2010, 7: 5-10.1186/1550-2783-7-5.PubMed CentralView ArticlePubMedGoogle Scholar
- Jia F, Yue M, Chandra D, Keramidas A, Goldstein PA, Homanics GE, Harrison NL: Taurine is a potent activator of extrasynaptic GABA(A) receptors in the thalamus. J Neurosci. 2008, 28: 106-115. 10.1523/JNEUROSCI.3996-07.2008.View ArticlePubMedGoogle Scholar
- Seidl R, Peyrl A, Nicham R, Hauser E: A taurine and caffeine-containing drink stimulates cognitive performance and well-being. Amino acids. 2000, 19: 635-642. 10.1007/s007260070013.View ArticlePubMedGoogle Scholar
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