Fig. 4
PA increases C2C12 myoblast mTOR signaling and MPS. Legend: Data are presented as means ± standard error. All data were obtained from 2–3 independent experiments (n = 6–11 wells per condition). Significant between-treatment differences (p < 0.001) existed for p-p70s6k (Thr389) and MPS levels as determined by independent samples t-tests (denoted by different superscript letters). Panel a: C2C12 myoblasts were serum starved for 18 h and then stimulated for 30 min with 30 μM PA, or PBS as a control condition. Measurements of mTOR signaling were assessed by evaluating the phospho to total ratio (P:T) for p-p70s6k (Thr389) and total p70S6k. Panel b: C2C12 myoblasts were treated as described in panel a and measurements of protein synthesis were performed by assessing puromycin incorporation during the final 30 min of a 60 min stimulation period. The Western blot membranes were stained with Coomassie Blue to verify equal loading of protein in all lanes