Volume 5 Supplement 1

Proceedings of the Fifth International Society of Sports Nutrition (ISSN) Conference and Expo

Open Access

Beta-alanine supplementation and high-intensity interval training augments metabolic adaptations and endurance performance in college-aged men

  • Abbie E Smith1Email author,
  • Ashley A Walter1,
  • Kristina L Kendall1,
  • Jennifer L Graef1,
  • Christopher M Lockwood1,
  • Jordan R Moon1,
  • Travis W Beck1,
  • Joel T Cramer1 and
  • Jeffery R Stout1
Journal of the International Society of Sports Nutrition20085(Suppl 1):P5


Published: 17 September 2008


A randomized, double-blind, placebo-controlled study was conducted to evaluate the effects β-alanine supplementation and high-intensity interval training (HIIT) on endurance performance.


Forty-six college-aged men (Age: 22.2 ± 3.3 yrs, VO2peak: 42.6 ± 6.2 ml·kg·min-1, 3.3 ± 0.6 l·min-1) volunteered to participate. In a random fashion, all subjects were placed into one of three groups: placebo (PL – 16.5 g of flavored dextrose powder per packet; n = 18), β-alanine (BA – 1.5 g β-alanine plus 15 g of flavored dextrose powder per packet; n = 18) or control (n = 10) groups. Each treatment group ingested one packet 4 times per day (total of 6 g/day) for the first 21-day adaptation phase, followed by 2 times per day (3 g/day) for the subsequent 21 days. All participants performed a continuous VO2peak test on a cycle ergometer (Corval Lode, Gronigen, the Netherlands) which was further used to establish ventilatory threshold (VT), and total time to exhaustion (VO2TTE, seconds) at pre-, mid- and post-testing. Total work done (TWD) was also measured, calculated from the total time (T; seconds) completed at a workload corresponding to 110% of their maximal power output (watt, W) determined from the VO2peak test [TWD (kJ) = (T × W)/1000]. Following initial testing, all participants in the BA and PL groups engaged in a 3 week supplementing and training adaptation phase. Each training session in the adaptation phase consisted of 5 bouts of a 2:1 minute cycling work to rest ratio, introduced in an undulating progression starting at 90% VO2peak power output and reaching 110%. The second 3 week training phase progressed, reaching intensities up to 115% of VO2peak. Body composition was assessed using air displacement plethysmography (Bod Pod®) at pre- mid- and post-testing. Separate one-way analyses of covariance were used to identify and group (BA vs. PL. vs. CON) × time (Mid- vs. Post-) interactions, adjusting mean post-test values for differences in the mid-test scores, due to the supplementing and training adaptation phase.


There was a significant difference among all post-test GXT variables (VO2peak, VO2TTE, and VT) and TWD, after adjusting for the mid-test adaptation values (p ≤ 0.000). However, there were no differences between treatment group means. Individual responses indicated a greater number of the BA participants improving in VO2peak (83%) and VO2TTE (72%) performance over the PL group (61% and 56%, respectively). Furthermore, bonferroni-corrected post-hoc pairwise comparisons indicated the significant increases in TWD were greater for the BA group than the CON (p = 0.029). There were no significant changes in body composition following training and supplementing.


Three weeks of combined β-alanine supplementation and HIIT, following a 21-day β-alanine loading and HIIT adaptation phase, significantly improves aerobic performance. The improvements in performance may be attributed to a greater reliance on aerobic metabolism due to chronic adaptations to HIIT, in combination with an improved muscle buffering capacity as a result of an increase in intramuscular carnosine levels.



The authors would like to thank FSI Nutrition, 2132 South 156th Circle, Omaha, NE http://www.fsinutrition.com and RunFast Promotions, 8790 Wendy Lane South, West Palm Beach FL, 33411 http://www.runfastpromotions.com for supporting and funding this research endeavor.

Authors’ Affiliations

FISSN Department of Health & Exercise Science, University of Oklahoma


© Smith et al; licensee BioMed Central Ltd. 2008

This article is published under license to BioMed Central Ltd.