Botanical supplements with flavonoids possess the ability to reduce inflammatory markers such as CRP, IL-6, and TNF-α. Also, they could potentially help reduce sugar-induced weight gain and facilitate weight loss. Diafin is a non-stimulant, botanical, weight loss product created from a blend of standardized Free-B-ring flavonoids and flavans from two plant extracts isolated from the Scutellaria genus of plants and the Acacia genus of plants. Flavonoids, specifically from the Scutellaria genus, have been used previously for anti-inflammatory and cardiovascular applications, and have been suggested to inhibit eicosanoid generating enzymes such as phospholipase A_2, cyclooxygenases, and lipoxygenases, while concomitantly reducing prostanoids and leukotrienes. However, the exact mechanism in which flavonoids induce an anti-inflammatory effect is unclear.

The purpose of this study was to determine the effects of eight weeks of daily ingestion of a botanical, anti-inflammatory, nutritional supplement combined with resistance training and an energy-controlled diet on body composition, muscular performance, and serum lipids, obesity hormones, and inflammatory markers.

Sedentary, obese women (n = 40) participated in a full-body resistance training program 3 days/week for 8 weeks while following an energy-restricted, low-glycemic diet and also ingested either 125 mg of a botanical, anti-inflammatory product (Diafin, Unigen Pharmaceuticals, Lacey, WA) or 125 mg of a cellulose placebo in a randomized, double blind, placebo-controlled fashion. Body composition, muscle performance, serum lipids, and inflammation and obesity markers were obtained at week 0 and after weeks 4 and 8. Data were analyzed by repeated measures ANOVA and are presented as means ± SD.

For body composition, there was a significant time main effect for body mass, BMI, and fat mass. Body mass (p < 0.001), BMI (p < 0.001), and fat mass (p = 0.034) all decreased significantly for both groups between weeks 0 and 8. For muscle performance, there was a significant time main effect for leg press and bench press strength as both strength variables increased in both groups between weeks 0 and 8 (p < 0.001). For serum lipids, there was a significant time main effect for TCHOL, LDL, and HDL. TCHOL (p = 0.004), LDL (p = 0.048), and HDL (p = 0.009) decreased between weeks 0 and 8. There was also a significant time main effect for leptin, which decreased significantly between week 0 and 8 (p = 0.019).

It is concluded that a full-body resistance training program, in combination with an energy-restricted, low glycemic diet: 1) promotes weight loss and strength gains, 2) improves total and LDL cholesterol, and 3) decreases circulating leptin levels in previously-sedentary, obese women.