Pain ratings from all 3 quadriceps locations (p<0.001); AST, ALT, CK, BUN/Cr ratio, UA (p<0.001); cortisol, testosterone, and CORT/TEST ratio (p<0.001) all demonstrated significant changes in both groups over time. The overall MANOVA analysis revealed a trend toward a significant group x time effect for all pain ratings (p=0.093) and cortisol, testosterone, CORT/TEST ratio (p=0.071), but no significant interaction MANOVA Wilks’ Lambda effects were seen for AST, ALT, CK, BUN/Cr ratio, UA (p=0.365). MANOVA univariate analysis revealed significant effects for v. medialis [¼] (p=0.003) and v. lateralis [½] (p<0.001) pain perceptions, AST, ALT, UA, cortisol (p<0.001), CK (p=0.001), BUN/Cr ratio (p=0.008), and cort/test ratio (p=0.042) in both groups over time in addition to trends for both groups over time for v. lateralis [¼] pain perception (p=0.12) and testosterone (p=0.08). A significant group x time linear effect was shown for v. medalis [¼] pain perception (p=0.035) with a trend toward a significant quadratic interaction for the v. lateralis [½] pain perception (p=0.053). A significant delta value was also shown based on v. medialis [¼] pain perception differences in group assignment (p=0.029). No significant group x time interaction was evident in pain perception from the v. lateralis [¼] (p=0.92). A significant group x time quadratic effect was shown for BUN/Cr ratio (p=0.048). No significant group x time interaction was evident for ALT (p=0.70), AST (p=0.92), CK (p=0.87), and UA (p=0.50). The MANOVA univariate analysis also revealed a significant group x time effect for cortisol (p=0.05) coupled with a more specific group x time significant cubic (p=0.009) and linear (p=0.016) interactions for cortisol.