Pain ratings from all 3 quadriceps locations (p<0.001); AST, ALT, CK, BUN/Cr ratio, UA (p<0.001); cortisol, testosterone, and cort/test ratio (p<0.001) all demonstrated significant changes in both groups over time, but the overall Wilks’ Lambda MANOVA analysis did not reveal a significant group x time effect for any pain ratings (p=0.199); AST, ALT, CK, BUN/Cr ratio, UA (p=0.605); cortisol, testosterone, CORT/TEST ratio (p=0.35). MANOVA univariate analysis revealed significant time effects for all locations of pain ratings (p<0.001); AST, UA (p<0.001); cortisol, testosterone (p<0.001); CK (p=0.003); and CORT/TEST ratio (p=0.022) in both groups in addition to trends for both groups over time for ALT (p=0.094) and BUN/Cr ratio (p=0.059). A significant group x time quadratic effect was shown for v. lateralis [¼] pain perception (p=0.024) with a trend toward a significant cubic interaction for the v. lateralis [½] pain perception (p=0.10). No significant group x time interaction was evident in pain perception from the v. medialis [¼] (p=0.24), but a delta value trend was shown based on v. medialis [¼] pain perception differences in group assignment (p=0.10). A significant group x time linear effect was shown for UA (p=0.014) along with a trend toward a significant linear interaction (p=0.067) and a significant delta value based on group assignment for ALT (p=0.005). A trend toward a significant group x time linear effect was shown for cort/test ratio (p=0.10). No significant group x time interactions were evident for cortisol (p=0.45) and testosterone (p=0.17).