Volume 10 Supplement 1

Proceedings of the Tenth International Society of Sports Nutrition (ISSN) Conference and Expo

Open Access

Effects of exercise and diet-induced weight loss on markers of inflammation II: impact on microRNA 21 and microRNA 146a expression and their regulatory role

  • S Simbo1,
  • A Roque-Andrade2,
  • B Lockard1,
  • C Baetge1,
  • S Mertens-Talcott2,
  • C Rasmussen1 and
  • R Kreider1
Journal of the International Society of Sports Nutrition201310(Suppl 1):P24


Published: 6 December 2013


Obesity has been associated with inflammation. However, the mechanisms are not well understood. The purpose of this study was to determine if exercise and diet-induced weight loss would affect markers of inflammation via the Phosphatase and Tensin homologue Deleted from Chromosome-10 (PTEN), TNF receptor-associated factor 6 (TRAF6), Phosphatidylinositol-3-kinase (PI3k), Protein Kinase B (AKT or PKB), Nuclear Factor kappa Beta (NF-kB) signaling pathway through the regulation of microRNA 21 and microRNA 146a expression.


Forty-five overweight and sedentary women (48.16±10.5 yr, 45.9±4.4% body fat, BMI 35.6±5.6 kg/m2) were randomized into a control group (C, n=18) or an exercise and diet-induced weight loss group (EX, n=27). Participants followed an energy-restricted diet (1,200 kcal/d for 1 week and 1,500 kcal/d for 11weeks; 30% CHO, 45% P, and 25% F) while participating in a circuit resistance-training (3d/wk) program. The resistance training program included 30 seconds of resistance exercise interspersed with 30 seconds of continuous movement (calisthenics). Whole blood samples were obtained at 0 and 12 wks and centrifuged immediately to obtain white blood cells buffy coat for mRNA isolation. The microRNA (21 and 146a) and mRNA of IL-6, TNF-α, (PTEN, TRAF6)/PI3k/AKT/NF-kB signaling pathway expression levels were measured in serum/WBC (buffy coat) by real-time RT-PCR and normalized using ΔΔCt formula with U6B as a normalization control for the microRNAs and Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an endogenous control for mRNAs. The ΔΔ C Baetge, B Lockard Ct formula, Ct represents the real time cycle number at which microRNA and mRNA probe fluorescence is exponential. Data were analyzed by MANOVA and presented as changes from baseline after 12 wks.


An overall significant MANOVA interaction was observed among EX and C groups (Wilks’ Lambda p<0.001). MANOVA univariate analysis revealed no significant interactions among groups in changes in microRNA 146a (EX -0.73±2.0; C -0.28±2.1, p=0.46); TRAF6 (EX –1.35±2.7; C -0.74±3.5, p=0.52); mRNA expression levels of PI3K (EX -2.4±4.5; C -1.8±2.9, p=0.66); AKT (EX -1.34±4.2; C -0.67±7.4, p=0.70); or, mRNA NF-kB (EX -1.6±3.2; C -0.73±3.2, p=0.40). Significant interactions were observed among groups in changes in microRNA 21 (EX -1.5±2.34; C 0.13±2.2, p=0.03); mRNA expression level of its target gene PTEN (EX -4.5±3.2; C -1.6±3.4, p=0.005); mRNA IL-6 (EX -2.8±3.6; C 2.8±2.2, p<0.001); and, mRNA TNF-α expression levels (EX -0.52±2.5; C 2.3±1.9, p<0.001). Exercise and diet-induced changes in mRNA IL-6 and mRNA TNF-α expression were positively and significantly correlated to changes in body weight (r=0.47, r=0.30), fat mass (r=0.48, r=0.31), and percent body fat (r=0.48, r=0.32), respectively.


Results of this study indicate that exercise and diet-induced weight loss affects molecular changes in circulating microRNAs, significantly affects microRNA 21 and its target gene PTEN, mRNA TNF-α, and mRNA IL-6 levels suggesting a anti-inflammatory response compared to a control group. These findings suggest that exercise and diet-induced weight loss is significantly associated with a reduction in inflammation. However, more research is needed to understand microRNA regulation associated with inflammation in response to exercise.



Supported by Curves International (Waco, TX)

Authors’ Affiliations

Exercise & Sport Nutrition Lab, Department of Health and Kinesiology, Texas A&M University
Department of Nutrition and Food Science, Texas A&M University


© Simbo et al; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.