Skeletal muscle metabolic gene response to carbohydrate feeding during exercise in the heat

Table 1 Primers and probes used for real-time PCR

Gene	Primer 1	Primer 2	Probe
GAPDH	TGTAGTTGAGGTCAATGAAGGG	ACATCGCTCAGACACCATG	AAGGTCGGAGTCAACGGATTTGGTC
MFN2	ATGCATCCCACTTAAGCAC	CCAGAGGGCAGAACTTTCTC	AGAGGCATCAGTGAGGTGCT
PGC-1α	ATAAATCACACGGCGCTCTT	TGAGAGGGCCAAGCAAAG	AGAGGCAGAGGCAGAAGG
UCP3	CAAAATCCGGGTAGTGAGGCT	TGACTCCGTCAAGCAGGTGTAC	CCCCCAAAGGCGCGGACAAC
GLUT4	TCTTCACCTTGGTCTCGGTGTTGT	CACGAAGCCAAAGATGGCCACAAT	ATGTGTGGCTGTGCCATCCTGATGA

GAPDH Glyceraldehyde 3-phosphate dehydrogenase, MFN2 mitofusin 2, PGC-1α peroxisome-proliferator- activated receptor-gamma co-activator 1 alpha, UCP3 uncoupling protein 3, GLUT4 glucose transporter 4.

ISSN: 1550-2783