Adjusted bone mineral content of lumbar spine, tibia proximal metaphysis, and tibia diaphysis. Bone mineral content of lumbar spine (A), tibia proximal metaphysis (B) and tibia diaphysis (C) adjusted to the 100 g body weight. The lumbar spine and tibia of each rat were isolated by dissection, and muscle and connective tissue were carefully removed. BMC was then measured by dual-energy X-ray absorptiometry. Vertical bars indicate the standard error. p value indicates statistical significant difference among dietary protein groups.