High-Protein Weight Loss Diets and Purported Adverse Effects: Where is the Evidence?
© A National Library of Congress Indexed Journal 2004
Received: 1 March 2004
Accepted: 9 May 2004
Published: 10 May 2004
Results of several recent studies show that high-protein, low-carbohydrate weight loss diets indeed have their benefits. However, agencies such as the American Heart Association (AHA) have some concerns about possible health risks. The purpose of this review is to evaluate the scientific validity of AHA Nutrition Committee's statement on dietary protein and weight reduction (St. Jeor ST et al. Circulation 2001;104:1869–1874), which states: "Individuals who follow these [high-protein] diets are risk for ... potential cardiac, renal, bone, and liver abnormalities overall. Simply stated, there is no scientific evidence whatsoever that high-protein intake has adverse effects on liver function. Relative to renal function, there are no data in the scientific literature demonstrating that healthy kidneys are damaged by the increased demands of protein consumed in quantities 2–3 times above the Recommended Dietary Allowance (RDA). In contrast with the earlier hypothesis that high-protein intake promotes osteoporosis, some epidemiological studies found a positive association between protein intake and bone mineral density. Further, recent studies studies suggest, at least in the short term, that RDA for protein (0.8 g/kg) does not support normal calcium homeostasis. Finally, a negative correlation has been shown between protein intake and systolic and diastolic blood pressures in several epidemiological surveys. In conclusion, there is little if any scientific evidence supporting above mentioned statement. Certainly, such public warnings should be based on a thorough analysis of the scientific literature, not unsubstantiated fears and misrepresentations. For individuals with normal renal function, the risks are minimal and must be balanced against the real and established risk of continued obesity.
Keywordshigh-protein diets adverse effects American Heart Association
Certainly, living organisms thrive best in the milieu and on the diet to which they were evolutionarily adopted. From all indications, Homo sapiens sapiens (anatomically modern humans) has remained biologically unchanged during at least the last 50,000 years.  It was not until some 10,000 years ago that the transition from a roaming hunter and gatherer to a stationary farmer began. Consequently, our diet has become progressively more divergent from those of our ancient ancestors. The typical Paleolithic diet compared with the average modern American diet contained 3 to 4 times more protein.
It is implausible that an animal that adapted to a high protein diet for 5 million years suddenly in 10,000 years becomes a predominant carbohydrate burner. Indeed, counter to the current US Dietary Guidelines which promotes diet high in complex carbohydrates, recent clinical investigations support the efficacy of high-protein diets for weight loss/fat loss, as well as for improved insulin sensitivity and blood lipid profiles. Thus, the popularity of high-protein diets for weigh loss is unquestionable. However, there are always some concerns about high-protein diets.
In 2001, the American Heart Association (AHA) Nutrition Committee published statement on dietary protein and weight reduction. According to this statement, "Individuals who follow these [high-protein] diets are risk for ... potential cardiac, renal, bone, and liver abnormalities overall. However, it should be noted that there is little if any evidence supporting these contentions. Thus, this review deals with the relationship between protein intake and renal function, bone health, blood pressure, heart disease and liver function. Also, effects of very-low carbohydrate diet on lean body mass loss are discussed.
Protein Intake and Renal Function
Despite its role in nitrogen excretion, there are presently no data in the scientific literature demonstrating the healthy kidney will be damaged by the increased demands of protein consumed in quantities above the Recommended Dietary Allowance (RDA). Furthermore, real world examples support this contention since kidney problems are nonexistent in the bodybuilding community in which high-protein intake has been the norm for over half a century. Recently, Walser published comprehensive review on protein intake and renal function, which states: "it is clear that protein restriction does not prevent decline in renal function with age, and, in fact, is the major cause of that decline. A better way to prevent the decline would be to increase protein intake. there is no reason to restrict protein intake in healthy individuals in order to protect the kidney."
The study by Poortmans and Dellalieux investigated body-builders and other well-trained athletes with high- and medium-protein intake, respectively.  The athletes underwent a 7-day nutrition record analysis as well as blood sample and urine collection to determine the potential renal consequences of a high protein intake. The data revealed that despite higher plasma concentration of uric acid and calcium, bodybuilders had renal clearances of creatinine, urea, and albumin that were within the normal range. To conclude, it appears, at least in the short term, that protein intake under 2.8 g/kg does not impair renal function in well-trained athletes.
More recently, Knight et al. determined whether protein intake influences the rate of renal function change in women over an 11-year period. 1624 women enrolled in the Nurses' HealthStudy who were 42 to 68 years of age in 1989 and gave bloodsamples in 1989 and 2000. Ninety-eight percent of women werewhite, and 1% were African American. In multivariate linear regression analyses, highproteinintake was not significantly associated with change in estimated glomerular filtration rate(GFR) in women with normal renal function (defined as an estimatedGFR ≥80 mL/min per 1.73 m2). Thus, the authors concluded that high protein intake does not seem tobe associated with renal function decline in women with normalrenal function. As pointed out by Lentine and Wrone, the generalizability of these findings is limited by sampling characteristics to white mid-adulthood, but this limitation is overshadowed by strong internal validity grounded in a large sample size, prospective outcomes ascertainment, and adjustment for multiple covariates.
Chronic Renal Failure
Historically, dietary protein restriction has been recommend as a therapeutic approach for delaying the progression of chronic renal failure (CRF). However, as pointed out by Ikizler, it is important to reassess the applicability of this approach. Indeed, the results of the largest randomised clinical trial, The Modification of Diet in Renal Disease (MDRD), did not demonstrate a benefit of dietary protein restriction on progression of renal disease. Further, CRF patients have been shown to require a protein intake of 1.4 g/kg/day to maintain a positive or neutral nitrogen balance during nondialysis days, and even this intake may not be adequate for dialysis days.
According to American Diabetes Association (ADA), there is no evidence to suggest that usual protein intake (15–20% of total calories) should be modified if renal function is normal. The long-term effects of consuming >20% of energy as protein on the development of nephropathy has not been determined, and therefore ADA nutritionists felt it may be prudent to avoid protein intakes >20% of total daily energy. More recently, the metabolic effects of a high-protein diet were compared with those of the prototypical healthy (control) diet, which is currently recommended to persons with type 2 diabetes. The ratio of protein to carbohydrate to fat was 30:40:30 in the high-protein diet and 15:55:30 in the control diet. The high-protein diet resulted in a 40% decrease in the mean 24-h integrated glucose area response. Further, glycated hemoglobin decreased 0.8% and 0.3% after 5 weeks of the high-protein and control diets, respectively. Finally, fasting triacylglycerol was significantly lower after the high-protein diet than after the control diet. The authors concluded that a high-protein diet lowers blood glucose postprandially in persons with type 2 diabetes and improves overall glucose control. Cleary, longer-term studies are necessary to determine the total magnitude of response and possible adverse effects.
Protein Intake and Bone Health
Increasing dietary protein increases urine calcium excretion such that for each 50 g increment of protein consumed, and extra 60 mg of urinary calcium is excreted. It follows that the higher the protein intake, the more urine calcium is lost and the more negative calcium balance becomes. Since 99% of the body's calcium is found in bone, one would hypothesize that high protein induced hypercalciuria would results in high bone resorption and increased prevalence of osteopenia or osteoporotic-related fractures.
However, the epidemiological and clinical data addressing this hypothesis are controversial. In fact, some epidemiological studies found a positive association between protein intake and bone mineral density (BMD).[9, 37, 38] Further, there is growing evidence that a low protein diet has a detrimental effect on bone. For example, Kerstetter et al. reported that in healthy young women, acute intakes of a low-protein diet (0.7 g protein/kg) decreased urinary calcium excretion with accompanied secondary hyperparathyroidism. The etiology of the secondary hyperparathyroidism is due, in part, to a significant reduction in intestinal calcium absorption during a low protein diet.
In a recent short-term intervention trial, Kerstetter et al. evaluated the effects of graded levels of dietary protein (0.7, 0.8, 0.9, and 1.0 g protein/kg) on calcium homeostasis. Secondary hyperparathyroidism developed by day 4 of the 0.7 and 0.8 g protein/kg diets (due to the decreased intestinal calcium absorption), but not during the 0.9 or 1.0 g protein/kg diets in eight young women. There were no significant differences in mean urinary calcium excretion over the relatively narrow range of dietary protein intakes studied, although the mean value with the 0.7-g/kg intake was lower than that with the 1.0 g/kg intake by 0.25 mmol (10 mg). According to authors of this study, the lack of change may be due to the small sample and the inherent variability in urinary calcium excretion. Similarly, when Giannini et al. restricted dietary protein to 0.8 g protein/kg, they observed an acute rise in serum parathyroid hormone (PTH) in 18 middle-aged hypercalciuric adults. Taken together, both of studies suggest, at least in the short term, that the RDA for protein (0.8 g/kg) does not support normal calcium homeostasis.
Furthermore, dietary protein increases circulating IGF-1, a growth factor that is thought to play an important role in bone formation. Indeed, several studies have examined the impact of protein supplementation in patients with recent hip fractures. For example, Schurch et al. reported that supplementation with 20 g protein/day for 6 months increased blood IGF-levels and reduced the rate of bone loss in the contralateral hip during the year after the fracture. More recently, the Cochrane-review assessed the effects of nutritional interventions in elderly people recovering from hip fracture. Seventeen randomised trials involving 1266 participants were included. According to reviewers, the strongest evidence for the effectiveness of nutritional supplementation exists for oral protein and energy feeds, but the evidence is still weak.
Moreover, many of these early studies that demonstrated the calciuric effects of protein were limited by low subject numbers, methodological errors and the use of high doses of purified forms of protein. Indeed, the recent study Dawson-Hughes et al. did not confirm the perception that increased dietary protein results in urinary calcium loss. According to Dawson-Hughes et al., "Theconstellation of findings that meat supplements containing 55 g/d protein, when exchanged for carbohydrate did not significantlyincrease urinary calcium excretion and were associated withhigher levels of serum IGF-I and lower levels of the bone resorption marker, N-telopeptide, together with a lack of significant correlationof urinary N-telopeptide with urinary calcium excretion in thehigh protein group (in contrast to the low protein) point tothe possibility that higher meat intake may potentially improvebone mass in many older men and women."
Finally, the cross-cultural and population studies that showed a positive association between animal-protein intake and hip fracture risk did not consider other lifestyle or dietary factors that may protect or increase the risk of fracture. It is of some interest that the author of the most cited paper favoring the earlier hypothesis that high-protein intake promotes osteoporosis no longer believes that protein is harmful to bone. In fact, he concluded that the balance of the evidence seems to indicate the opposite.
Protein Intake and Blood Pressure
The AHA Nutrition Committee suggests that high-protein intake may increase blood pressure. However, there is no scientific evidence supporting this contention. In fact, a negative correlation has been shown between protein intake and systolic and diastolic blood pressures in several epidemiological surveys analyzed by Obarzanek et al. For example,
Honolulu Heart Study. In this study of 6,406 Japanese-American men, a negative relationship was observed between systolic and diastolic blood pressures and the amount protein consumed.
Chinese Study. In this investigation of 2,672 adults men and women, a negative relationship was found between systolic pressure and the amount of animal protein consumed.
MRFIT Study. Based on 11,342 adult men, investigators observed a negative relationship between systolic blood pressure and the amount of total protein consumed.
In both normotensive and hypertensive rats, increasing the dietary protein level enhances both urine and the amount of sodium excreted, although the mechanism behind these effects is unknown and still speculative. Interestingly, one study in human volunteers with a family history of hypertension has shown that a high-protein diet may counteract the adverse effects of excessive salt intake. For more information on protein intake and blood pressure, see the recent review by Debry.
Protein Intake and Heart Disease
Recent findings by Hu et al. suggests that replacing carbohydrates with protein may be associated with a lower risk of ischemic heart disease. This result is consistent with evidence from metabolic studies that replacement of dietary carbohydrate with protein has favorable effect on plasma lipoprotein and lipid concentrations. However, because an increase in protein intake from animal products such as meats, dairy products, and eggs is often accompanied by increases in intakes of saturated fat and cholesterol, dietary advice to improve public health based on these findings should be made with caution.
Recent novel approaches have shown that glucose and lipid intake may induce an increase in the generation of reactive oxygen species (ROS) and oxidative stress. For example, Mohanty et al. produced evidence that all three major macronutrients induce an increase in ROS generation. However, their data also show that different nutrients produce distinct patterns of stimulation of ROS generation after their intake. Of the three nutrients, glucose induced the greatest ROS generation, followed in decreasing order by fat (cream) and by protein (casein). The detriment of oxidative stress is that it may damage proteins and lipids, the latter through lipid peroxidation. Lipid peroxidation of LDL-C particles is an essential step in the development of atherosclerosis.
Protein Intake and Liver Function
AHA Nutrition Committee suggests that high-protein intake may have detrimental effects on liver function. However, there is no scientific evidence whatsoever supporting this contention. Protein is needed not only to promote liver tissue repair, but also to provide lipotropic agents such as methionine and choline for the conversion of fats to lipoprotein for removal from the liver, thus preventing fatty infiltration.
Rodents fed very high protein intakes have been found to exhibit morphological changes in the liver mitochondria, which could be pathological. However, Jorda et al. reported that the liver responds to the high-protein diet by a proliferation of normally functioning mitochondria. Further, the branched-chain amino acids to aromatic amino acids ratio was also increased, indicating the absence of hepatic failure in these animals. The authors concluded that "the increased protein content of diet induced rapid increases in several characteristics of hepatocytes ... The results presented here constitute a good example of how the hepatocyte adapts to a continuing metabolic stress."
Further, protein catabolism is increased in liver disease and may be exacerbated by inadequate protein in the diet. Unless there is encephalopathy (vide infra), the diet should provide high-quality protein in the amount of 1.5 to 2 g/kg. In alcoholic liver disease, a high-calorie, high-protein diet has been shown to improve hepatic function and reduce mortality. In one study, this was achieved by providing a regular diet plus supplements of 60 g/day of protein and 1600 kcal/day for the first 30 days and followed by supplements of 45 g/day of protein and 1200 kcal/day for the next 60 days.
Finally, the role of protein restriction in patients with chronic hepatic encephalopathy (HE) has been questioned recently as the efficacy of protein withdrawal in patients with HE has never been subjected to a controlled trial. According to Srivastava et al., "the emphasis in the nutritional management of patients with HE [hepatic encephalopathy] should not be on the reduction of protein intake. Instead, the goal should be to promote synthesis by making available ample amounts of amino acids, while instituting other measures to reverse the ongoing catabolism."
Effects of Very-Low-Carbohydrate Diet on Lean Body Mass
According to the AHA Nutrition Committee, "Some popular high-protein/low-carbohydrate diets limit carbohydrates to 10 to 20 g/d, which is one fifth of the minimum 100 g/day that is necessary to prevent loss of lean muscle tissue." Clearly, this is an incorrect statement since catabolism of lean body mass is reduced by ketones, which probably explains the preservation of lean tissue observed during very-low-carbohydrate diets.
For example, Volek et al. examined the effects of 6-week carbohydrate-restricted diet on total and regional body composition and the relationships with fasting hormones. Twelve healthy normal-weight men switched from their habitual diet (48% carbohydrate) to a carbohydrate-restricted diet (8% carbohydrate) for 6 weeks and 8 men served as controls, consuming their normal diet. Subjects were encouraged to consume adequate dietary energy to maintain body mass during intervention.
Fat mass was significantly decreased (-3.4 kg) and lean body mass significantly increased (+1.1 kg) at week 6. However, there were no significant changes in composition in the control group. The Authors concluded that a carbohydrate-restricted diet resulted in a significant reduction in fat mass and a concomitant increase in lean body mass in normal-weight men. They hypothesized that elevated β-hydroxybutyrate concentrations may have played a minor role in preventing catabolism of lean tissue but other anabolic hormones were likely involved (e.g., growth hormone).
Oddly, the AHA Nutrition Committee ignores the fact that energy restriction increases protein requirements. It has been know for about a half century that inadequate energy intake leads to increased protein needs, presumably because some of the protein normally used to synthesize both functional (enzymatic) and structural (tissue) protein is utilized for energy under these conditions. For example, Butterfield has shown that feeding as much as 2 g protein/kg/day to men running 5 or 10 miles per day at 65% to 75% of their VO2max is insufficient to maintain nitrogen balance when energy intake is inadequate by as little as 100 kcal/day. Thus, when trying to lose weight, it is important to keep protein levels moderately high. The reduction in calories needed to lose weight should be at the expense of saturated fats and carbohydrates, not protein.
It is clear that the American Heart Association Nutrition Committee's statement on dietary protein and weight reduction contains misleading and incorrect information. Certainly, such public warnings should be based on a thorough analysis of the scientific literature, not unsubstantiated fears and misrepresentations. For individuals with normal renal function, the risks are minimal and must be balanced against the real and established risk of continued obesity.
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