The acute effects of caffeine intake on time under tension and power generated during the bench press movement

Background The ability to generate high levels of power is one of the key factors determining success in many sport disciplines. Although there are studies confirming ergogenic effects of caffeine (CAF) on different physical and mental abilities, much controversy remains about its influence on power. The main goal of this study was to assess the effects of caffeine supplementation on time under tension (TUT) and the number of performed repetitions (REP). The second objective was to determine the effects of CAF supplementation on power (P) and movement velocity (V) during the bench press movement. Additionally the authors evaluated whether CAF has a significant effect on velocity of the bar in the eccentric (ECC) phase (VEMEAN) of the bench press movement. Methods The study included 20 men (20–31 yrs., 87.3 ± 7.7 kg) with at least 2 years of experience in resistance training. The study participants were divided randomly into two groups: the supplemented group ingested caffeine before exercise (GCAF), while the control group was given a placebo (GCON). The exercise protocol consisted of performing the bench press movement with a load equal to 70%1RM with maximal possible velocity (X/0/X/0). The experimental sets were performed to momentary muscular failure. Results The repeated measures ANOVA between the GCAF and GCON groups revealed statistically significant differences in 2 variables. Post-hoc tests demonstrated statistically significant differences in TUT when comparing the group supplemented with caffeine (13.689 s GCAF) to the one ingesting a placebo (15.332 s GCON) at p = 0.002. Significant differences were also observed in mean velocity during the eccentric phase of movement (0.690 m/s in the GCAF to 0.609 in GCON with p = 0.002). There were no significant differences in generated power and velocity in the CON phase of the movement between the GCAF and GCON. Conclusions The main finding of the study is that CAF ingestion increases movement velocity of the bar in the eccentric phase of the movement, what results in shortening of the time under tension (TUT) needed for performing a specific number of repetitions, without decreasing power and velocity in the CON phase of the movement.


Background
Resistance training and power are significant components of conditioning programs in competitive sports. The ability to generate high levels of power has been indicated as a determinant of success in sports that require an optimal ratio of strength to velocity when performing a motor activity [1,2]. Optimization of resistance training and the pattern of adaptive changes related to the development of muscular strength and power have been the focus of interest of scientists from different fields of studies [3][4][5][6]. In addition to training, diet and supplementation also have a significant effect on adaptation and post-exercise responses [7][8][9][10][11][12]. To date, few supplements have been shown to have a direct ergogenic effect on physical capacity. Among them are caffeine, creatine monohydrate, sodium bicarbonate and beta alanine [13]. Although studies have confirmed the ergogenic effects of caffeine (CAF) in many aspects, much controversy remains about its effects on the power generated by the upper limbs [14,15]. The most frequently consumed dose of caffeine during research with athletes ranges from 2 to 9 mg/kg body mass, ingested in the form of pills or capsules 30 to 90 min before exercise. Mechanisms responsible for ergogenic effects of caffeine are linked to the impact on various tissues, organs and systems, of the human body. In the central nervous system (CNS), CAF acts through interactions with adenosine receptors that influence the release of noradrenaline, dopamine, acetylcholine and serotonin [16][17][18][19] and, consequently, increase muscle tension [20]. Increased muscle activation can lead to a higher energy demand during exercise, thus leading to a faster depletion of energy substrates in muscle cells [21]. Caffeine can stimulate calcium release from the sarcoplasmic reticulum [22] and can also inhibit its reuptake [23]. Numerous studies have discussed the effects of caffeine intake on human physical fitness [24][25][26][27][28][29][30]. However, in regards to strength and power performance, the results of caffeine supplementation are ambiguous. Studies have shown faster neuromuscular conduction [31], increased motor unit activation [32,33], and increased number of repetitions (REP) in the bench press following caffeine intake 60 min before exercise, compared with a placebo [33,34]. Furthermore, Green et al. [35] failed to demonstrate significant differences in the number of REP between the placebo and CAF groups during the bench press and for the first and second set of the leg press exercise. No ergogenic effect of CAF on REP was also documented in a study by Grgic and Mikulic [36]. Considering the effects of CAF on the level of maximal strength, the results tend to be inconclusive. Goldstein et al. [18] demonstrated a significant effect of CAF supplementation on strength in a group of women. On the contrary, Astorino et al. [37], Beck et al. [15] did not find such an effect in a group of experienced strength athletes. One should emphasize that previous research on CAF supplementation and the level of strength and power, as well as the number of performed repetitions considered resistance exercises performed at volitional or maximal velocity (V) of the entire movement, without a precise control of movement tempo during the concentric (CON) and eccentric (ECC) phases. Movement tempo has been defined by seconds which correspond to individual movement cadences (ECC/ pause/ CON/pause). Value X for the concentric movement represents maximal movement velocity. Changes in movement tempo during resistance training impacts exercise volume, the level of generated force, muscle power, and the rate of muscle hypertrophy [38][39][40][41][42][43][44][45]. The number of REP performed at a specific tempo impacts total time under tension (TUT) in a particular set. TUT provides accurate information about the duration of resistance effort for a set and for the entire training session. Wilk et al. [44] demonstrated significant differences in TUT and REP between the 2/0/2/0 and 5/0/3/0, as well as 6/0/4/0 tempos despite using the same external load and exercise to momentary muscular failure. That study demonstrated, that a greater number of performed REP was not synonymous with longer TUT. Therefore, TUT is an indicator of the work performed by the muscles in both, the ECC and CON phases of the movement. TUT determines how long the resistance effort lasts regardless of the number of REP performed. Previous studies regarding the ergogenic effects of caffeine on exercise volume, number of performed repetitions or the level of generated power did not consider exercise cadence, nor particular movement phases and TUT. This may explain the divergent results of studies that have used caffeine intake before resistance exercise. Furthermore, studies have failed to analyse the effect of CAF during controlled or variable movement tempo or cadence and the effect of these factors on strength, muscle power and training volume. It has not been demonstrated whether the ergogenic effect of caffeine concerns the entire movement or particular phases of the movement (CON and ECC).
Therefore, the main objective of this study was to assess the effects of caffeine intake on time under tension, the number of performed repetitions, and to determine the influence of CAF on muscular power (P) and movement velocity (V) in the CON phase. An additional goal of the study was to demonstrate the effects of CAF supplementation on mean velocity in the ECC (VE MEAN ) phase of the bench press movement.

Methods
All testing was performed in the Strength and Power Laboratory at the Jerzy Kukuczka Academy of Physical Education in Katowice. The experiment was performed following a randomized crossover, placebo-controlled, double-blind design, where each participant performed a familiarization session with a 1RM test on one day, and two different experimental sessions a week apart. The study participants were divided randomly into two groups: the group that ingested caffeine (G CAF ) and the placebo group (G CON ). Sixty minutes before each exercise protocol, CAF (5 mg/kg b.m.) or a placebo (all-purpose flour) were administered orally. CAF was provided in the form of standard capsules containing 300 mg of CAF, as well as those specifically prepared for the research, containing 50 and 5 mg doses of CAF. The placebo was provided in identical capsules as CAF. All CAF and placebo capsules were manufactured by Olimp Labs. Prior to the start of the study, an interview was conducted regarding caffeine intolerance, or any other physiological reactions to cafe intake. None of the subjects reported side effects in response to the use of CAF. The participants were instructed to follow their general nutrition and exercise protocols. All subjects completed questionnaires regarding health history, habitual caffeine intake from coffee, tea, soft drinks, chocolate, sport drinks, and caffeine-containing OTC drugs one week prior to testing. They were selected on the basis of their habitual caffeine consumption (< 200 mg/week) as determined by the questionnaire. In addition, subjects were asked to refrain from heavy exercise and alcohol consumption for 48 h before testing and to abstain from consuming caffeine-containing foods and beverages for 7 days prior to, and throughout, the study.

Study participants
Twenty healthy strength trained men volunteered for the study after completing an ethical consent form (age = 25.7 ± 2.2 years, body mass = 87.3 ± 7.7 kg, bench press 1RM = 102,3 ± 8.5 kg; data presented as mean ± standard deviation [SD]) with a least one year of resistance training experience (2.3 ± 0.63 years; mean ± standard deviation [SD]). All study participants were over 18 years old. The participants were allowed to withdraw from the experiment at any moment, and were free of any pathologies or injuries. The study protocol was approved by the Bioethics Committee for Scientific Research, at the Academy of Physical Education in Katowice, Poland according to the ethical standards of the Declaration of Helsinki, 1983.

Familiarization session and one repetition maximum test
A familiarization session preceded the one repetition maximum testing. These two objectives were separated by a 15 min recovery period. The participants arrived at the laboratory at the same time of day as the upcoming experimental sessions (in the morning between 09:00 and 11:00 am) and cycled on an ergometer for 5 min at an intensity that resulted in a heart rate of around 130 bpm, followed by a general upper body warm-up. Next, the participants performed 15, 10, and 5 repetitions of the bench press exercise using 20, 40, and 60% of their estimated 1RM with a 2/0/X/0 cadence. The participants then executed single repetitions using a volitional cadence with a 5 min rest interval between successful trials. The load for each subsequent attempt was increased by 2.5 kg, and the process was repeated until failure. Hand placement on the barbell was individually selected with a grip width on the barbell of 150% individual biacromial distance (BAD) [46,47]. BAD was determined by palpating and marking the acromion with a marker, and then measuring the distance between these points with a standard anthropometric tape [48]. The positioning of the hands was recorded to ensure consistent hand placement during all testing sessions. No bench press suits, weightlifting belts, or other supportive garments were permitted.

Experimental sessions
Two testing sessions were used for the experimental trials. The grip width was marked on the barbell with thin strips of athletic tape at the start of each session. The general and specific warm-up for the experimental sessions was identical to the one used for the familiarization session. After the warm up, participants started the main examinations and performed one set of the bench press to momentary failure with a load of 70%1RM. The eccentric and concentric phases were performed at maximal possible velocity (X/0/X/0). All repetitions were performed without bouncing the barbell off the chest, without intentionally pausing at the transition between the eccentric and concentric phases, and without raising the lower back off the bench. The interval between two stages of the experiment was 7 days. During the experimental trials the participants were encouraged to perform at maximal engagement according to the recommendations by Brown and Weir [49]. A linear position transducer system "Tendo Power Analyzer" (Tendo Sport Machines, Trencin, Slovakia) was used for the evaluation of bar velocity. The Tendo Power Analyzer is a reliable system for measuring movement velocity and power [50][51][52][53][54][55][56]. The system consists of a velocity sensor connected to the load by a kevlar cable which, through an interface, instantly transmits the vertical velocity of the bar to a specific software installed in the computer (Tendo Power Analyzer Software 5.0). The measurement was made independently in each repetition and automatically converted into the values of power (max, mean), concentric velocity (max, mean) and eccentric velocity (mean). All familiarization and experimental sessions were recorded by means of a Sony camera (Sony FDR191 AX53). Time under tension was obtained manually from the recorded data using slow speed playback (1/5 speed). In order to ensure the reliability of manual data collection, four independent persons made the data analysis from the Sony camera. There were no significant differences in the TUT [s] between the data collected by 4 evaluators. All participants completed the described testing protocol. The procedures were performed in identical environmental conditions, with an air temperature of 19.2°C and humidity of 58% (Carl Roth hydrometer, Germany).
The following variables were registered:

Statistical analysis
The Shapiro-Wilk, Levene and Mauchly's tests were used in order to verify the normality, homogeneity and sphericity of the sample data variances. Verification of differences between the G CAF and G CON after caffeine and placebo supplementation was performed using ANOVA with repeated measures. Effect sizes (Cohen's d) were reported where appropriate. Parametric effect sizes were defined as large for d > 0.8, as moderate between 0.8 and 0.5, and as small for < 0.5 [57][58][59]. The statistical significance was set at p < 0.05. All statistical analyses were performed using Statistica 9.1 and Microsoft Office, and were presented as means with standard deviations.

Results
The repeated measures ANOVA between the G CAF and G CON after caffeine and placebo supplementation revealed statistically significant differences between groups in 2 variables (Tables 1, 2). An appropriate formula for comparing means in two groups of equal size is presented below: where n is the number of subjects required for each group, d is the standardized difference and c p,power is a constant defined by the values chosen for the P value and power. The number of participants required in each trial to detect a standardized difference of 0.87 with 80% power using a cutoff for statistical significance of 0.05 is as follows: n ¼ 2 0; 87 2 xc 0;05;80% ¼ 2 0; 756 x7; 9 ¼ 2; 39x7; 9 Post-hoc tests revealed statistically significant differences for TUT [s], when comparing the CAF group (13.689 s) to the control group (15.332 s), at p = 0.002. Similar, statistically significant differences were observed for eccentric velocity: from 0.690 m/s in the G CAF to 0.609 in G CON at p = 0.002.

Discussion
The main finding of the study was that CAF supplementation has a significant effect on TUT [s], with no significant changes in the number of performed REP [n]. The ingestion of CAF led to a significant decrease in TUT during the explosive bench press exercise between the CAF group (G CAF ) and the placebo group (G PLA ). To date, no scientific study has analyzed the effect of caffeine on TUT. Despite significant differences in TUT between G PLA and G CAF , it was not related to the number of performed REP. The evaluations showed no statistical significance in REP (G CAF 16.6 ± 1.56 vs G PLA 15.9 ± 1.69). This is inconsistent with previous findings [33,35,60,61]. Duncan et al. [34] demonstrated an increase in exercise volume using an external load of 60%1RM, following the use of 5 mg of CAF/kg/ b.m. In the next years, Duncan et al. [33,60] also confirmed a significant effect of CAF intake on the number of performed REP, exercise volume and increase in muscle activity. The results of our study are partially consistent with those published by Goldstein et al. [18], who failed to find the effect of CAF supplementation on the number of performed REP. A similar pattern was observed in a study by Williams et al. [14], Green et al. [35], Astorino et al. [37], Richardson and Clarke [62]. However, one should stress that most studies that have analyzed the effect of CAF intake on exercise volume, both those that have demonstrated a significant effect on exercise capacity [33,34,37,60,63] and those which did not confirm such effects [14,62], evaluated exercise volume using the number of REP or tonnage. No previous studies have taken into account movement tempo and TUT, during resistance exercises. According to Wilk et al. [44] TUT is a more accurate and credible indicator of work performed compared to the number of performed REP. These findings were also  confirmed in our study, which showed a significant decline in TUT between the G CAF and G CON groups (13,68 ± 1346 vs 15,33 ± 1698 s) with no significant changes in the number of REP. The TUT determines how long the resistance effort lasts regardless of the number of REP performed. A decreased values of TUT in the CAF group may have resulted from the increased muscle tension generated during the movement in the CAF group. CAF leads to higher activation of motor units [33] and higher MVIC [20,32]. Increased muscle activation can lead to a higher energy demand during exercise, thus leading to a faster depletion of energy substrates in muscle cells [21], which may partially explain a decline in TUT in the G CAF group. However, the effect of increased muscle tension following CAF intake did not modify the power generated during the CON phase of the movement. The experiment did not demonstrate significant changes in P MEAN , P MAX and V MEAN , V MAX , despite a significant shortening of TUT in the G CAF group. The lack of significant differences in P and V indicates that the decline in TUT in the G CAF group is not related to the level of generated power and velocity in the CON phase of the movement. Importantly, the experiment showed a significant increase in velocity but only in the ECC phase of the movement (VE MEAN ). A significant increase in ECC velocity (G CAF 0.690 ± 0.08 ms vs G PLA 0.609 ± 0.05 ms) with a presumable increase in muscle tension in G CAF can partially explain the decline in TUT. Cole et al. [64] and other authors suggested that caffeine impacts mechanoreceptors feedback, such as the Golgi apparatus and class III/IV muscle afferents [64][65][66]. Caffeine may alter feedforward information and may alter how information from either feedforward/feedbackward mechanism is processed centrally [67]. Despite the increase in velocity of the ECC movement, it did not lead to significant changes in velocity and power generated during the CON phase of the movement. Cronin et al. [68] demonstrated that muscle power generated during the CON movement depends on the effective use of the stretchshortening cycle (SSC). The SSC cycle leads to the release of the energy stored in the ECC phase in order to maximize power generated during the CON phase. The same author [68] demonstrated that performing an ECC contraction before the CON phase impacts the generated power compared to performing only the concentric phase of the movement. A faster tempo of the entire movement cycle without a pause between the ECC and CON phases increases activation and more effective utilization of the SSC cycle [69]. However, to date, it remains unclear whether the efficiency of SSC depends on the velocity in the ECC phase and whether the SSC is susceptible to CAF supplementation. Although in this study VE MEAN was higher in G CAF (0.690 ± 0.08 ms vs 0.609 ± 0.05 ms), this change did not lead to a more effective use of the energy accumulated for explosive performance of the CON phase.
Therefore, it can be speculated that the change in VE MEAN and CAF intake do not lead to a more effective use of the SSC cycle. No increase in both mean (V MEAN ; P MEAN ) and maximal (V MAX ; P MAX ) values of CON movement with declining TUT questions the validity of CAF intake before high-intensity anaerobic exercise with a duration of between ten and twenty seconds. A limitation of the present study pertains to the lack of assessments related to CAF intolerance in the tested athletes. However it should be noted that before and during the experiment no study participant reported any side effects from ingesting caffeine.

Conclusion
The results of the present study indicate that the use of CAF before exercise does not have a significant effect on the generated power and velocity of the CON phase of the movement. CAF ingestion increases movement velocity of the bar in the eccentric phase of the movement, what results in shortening of the time needed for performing a specific number of repetitions, without decreasing power and velocity of the CON phase of the movement. Our findings indicate that the value of TUT may be a more accurate and credible indicator of work performed during a resistance training session, compared to the number of performed repetitions and, consequently, may be a new variable used in the analysis of ergogenic aids on exercise capacity in athletes.