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Table 2 Results of the within-run and between-run precision and accuracy evaluation for the analysis of carnosine (CAR) and anserine (ANS) in plasma using LC-MS/MS

From: Ergogenic effect of pre-exercise chicken broth ingestion on a high-intensity cycling time-trial

Component Theoretical concentration (nM) Mean concentration ± SD (nM) Precision, RSD (%) Accuracy (%)
CAR 50 a 54.7 ± 2.3 4.2 9.4
50 b 54.4 ± 2.0 3.7 8.7
500 a 487.5 ± 20.0 4.1 −2.5
500 b 491.8 ± 17.1 3.5 −1.6
5000 4328.1 ± 190.7 4.4 − 13.4
5000 b 4419.0 ± 257.1 5.8 −11.6
ANS 50 a 53.8 ± 2.4 4.4 7.6
50 b 53.3 ± 2.4 4.6 6.6
500 a 483.7 ± 21.2 4.4 −3.3
500 b 499.1 ± 25.8 5.2 −0.2
5000 4307.6 ± 116.6 2.7 −13.8
5000 b 4509.2 ± 322.1 7.1 −9.8
  1. Concentrations of 50, 500 and 500 nM correspond with 11.3, 113.1 and 1131.2 ng mL− 1 of CAR and 12.0, 120.1 and 1201.3 ng mL− 1 of ANS, respectively; acceptance criteria: accuracy: ≥ 10 to < 100 ng mL− 1: − 30 to + 10%, ≥ 100 ng mL− 1: − 20 to + 10%, within-run precision (RSDmax): ≥ 10 to < 100 ng mL− 1: 15.0%, ≥ 100 ng mL− 1: 10%; between-run precision: ≥ 10 to < 100 ng mL− 1: 23%, ≥100 ng mL− 1: 16% [19].
  2. Note: a Within-run accuracy and precision (n = 6); b Between-run accuracy and precision (n ≥ 3 per day, at least 3 analysis days); SD Standard deviation, RSD Relative standard deviation