Ergogenic effect of pre-exercise chicken broth ingestion on a high-intensity cycling time-trial

Table 2 Results of the within-run and between-run precision and accuracy evaluation for the analysis of carnosine (CAR) and anserine (ANS) in plasma using LC-MS/MS

Component	Theoretical concentration (nM)	Mean concentration ± SD (nM)	Precision, RSD (%)	Accuracy (%)
CAR	50 ^a	54.7 ± 2.3	4.2	9.4
	50 ^b	54.4 ± 2.0	3.7	8.7
	500 ^a	487.5 ± 20.0	4.1	−2.5
	500 ^b	491.8 ± 17.1	3.5	−1.6
	5000	4328.1 ± 190.7	4.4	− 13.4
	5000 ^b	4419.0 ± 257.1	5.8	−11.6
ANS	50 ^a	53.8 ± 2.4	4.4	7.6
	50 ^b	53.3 ± 2.4	4.6	6.6
	500 ^a	483.7 ± 21.2	4.4	−3.3
	500 ^b	499.1 ± 25.8	5.2	−0.2
	5000	4307.6 ± 116.6	2.7	−13.8
	5000 ^b	4509.2 ± 322.1	7.1	−9.8

Concentrations of 50, 500 and 500 nM correspond with 11.3, 113.1 and 1131.2 ng mL^− 1 of CAR and 12.0, 120.1 and 1201.3 ng mL^− 1 of ANS, respectively; acceptance criteria: accuracy: ≥ 10 to < 100 ng mL^− 1: − 30 to + 10%, ≥ 100 ng mL^− 1: − 20 to + 10%, within-run precision (RSD_max): ≥ 10 to < 100 ng mL^− 1: 15.0%, ≥ 100 ng mL^− 1: 10%; between-run precision: ≥ 10 to < 100 ng mL^− 1: 23%, ≥100 ng mL^− 1: 16% [19].
Note: ^a Within-run accuracy and precision (n = 6); ^b Between-run accuracy and precision (n ≥ 3 per day, at least 3 analysis days); SD Standard deviation, RSD Relative standard deviation

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