Effects of protein type and composition on postprandial markers of skeletal muscle anabolism, adipose tissue lipolysis, and hypothalamic gene expression

Table 1 Contents of each protein per the 0.19 g protein dose of each respective protein

Amino Acid	70 W/30E (mg)	50 W/50E (mg)	30 W/70E (mg)	WPC (mg)
Alanine	10	10	10	9
Arginine	8	8	9	6
Aspartic Acid	21	20	19	22
Cysteine	5	5	5	4
Glutamic Acid	31	29	26	34
Glycine	4	5	5	4
Histidine*	4	4	4	4
Isoleucine*†	11	11	10	12
Leucine*†	20	19	17	23
Lysine*	17	15	14	19
Methionine*	5	5	6	4
Phenylalanine*	8	9	9	7
Proline	12	11	9	17
Serine	12	12	11	12
Threonine*	11	10	9	12
Tryptophan*	3	3	3	3
Tyrosine	7	7	7	6
Valine*†	12	12	12	11
Total EAAs*	92	87	83	95
**Total BCAAs†***	43	41	38	46
M.W.	70 W/30E	50 W/50E	30 W/70E	WPC
(kDa)	(%)	(%)	(%)	(%)
<1.0	40	39	40	0
1.0 - 5.0	23	22	23	7
5.0 - 10.0	6	6	6	11
>10.0	30	32	32	82

As stated in the methods, rats were administered either WPC, 70 W/30E, 50 W/50E, or 30 W/70E at a human equivalent (eq.) dose of 10 g protein (equaled a true dose of 0.191 g of protein) dissolved in 1 ml of water via gavage feeding. While not stated in the methods, total protein was determined using the Dumas (N x 6.38) test method. Furthermore, amino acid concentrations (g/100 g) were determined using liquid chromatography. Finally, molecular weight (M.W.) distribution was determined by high-performance liquid chromatography size exclusion (HPLC-SEC), on an Agilent 1290 Infinity Quaternary LC System w/ TOSOH TSKgel G2000SW 7.5 mm ID × 30 cm (10 μm) column at a wavelength of 205 nm. Symbols: * indicates an essential amino acid; † indicates a branched chain amino acid (BCAA).

ISSN: 1550-2783