Figure 1

Effects of different protein feedings on skeletal muscle anabolism markers. Legend: Effects of each protein on gastrocnemius p-mTOR (Ser2481) (panel a), p-p70s6k (Thr389) (panel b), p-rps6 (Ser235/236) (panel c), p-4E-BP1 (Thr37/46) (panel d), and muscle protein synthesis (MPS) (panel e). Data are presented as means ± standard error (CTL n = 12–14 per bar, protein groups n = 6–8 per bar). One-way ANOVAs with a Fisher’s LSD post hoc test were performed and significant between-feeding differences are represented with different superscript letters (p < 0.05). Panel f: Example digital images of Akt-mTOR substrates of CTL rats and 70 W/30E-fed rats 180 min post-gavage. Panel g: Representative digital images of puromycin integration into muscle protein (SUnSET determination of MPS).