Figure 4

Chromatographic profile of HEE and the respective spectra of the prominent peaks. The experimental 5:100% water/methanol condition gradient, measured at a wavelength of 250 nm - 350 nm with the absorption spectra of UV/Vis prominent peaks (A) of spectra, and segmented (B) for each peak: Peak 1 - band “A” 271 nm, and band “B” = 334 nm; Peak 2 - band “A” 271 nm, and band “B” 336 nm; Peak 3 - band “A” 269 nm, and band “B” 334 nm; Peak 4 - band “A” 270 nm, and band “B” 335 nm.