Figure 4

Chromatographic profile of HEE and the respective spectra of the prominent peaks. The experimental 5:100% water/methanol condition gradient, measured at a wavelength of 250Â nm - 350Â nm with the absorption spectra of UV/Vis prominent peaks (A) of spectra, and segmented (B) for each peak: Peak 1 - band âAâ 271Â nm, and band âBâ = 334Â nm; Peak 2 - band âAâ 271Â nm, and band âBâ 336Â nm; Peak 3 - band âAâ 269Â nm, and band âBâ 334Â nm; Peak 4 - band âAâ 270Â nm, and band âBâ 335Â nm.