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Table 2 Proteins from myotubes identified by MALDI-TOF MS of spots after 2-DGE.

From: Creatine-induced activation of antioxidative defence in myotube cultures revealed by explorative NMR-based metabonomics and proteomics

Spot id Protein Sequence coveragea Matched peptidesb Scorec Theo. pId Theo. Mwe (kDa) Access keyf
High in CMH        
267 Vimentin 37 21 189 4.9 54 P20152
522 Malate dehydrogenase - cytoplasmic 21 6 65 6.2 37 Q6PAB3
667 Peroxiredoxin-4 26 6 73 6.8 31 O08807
680 Thioredoxin dependent peroxide reductase 45 9 98 5.9 28 P20108
High in Controls        
171 GRP75, 75 kDa glucose regulated protein precursor 16 10 76 5.8 74 P38674
941 GRP78, 78 kDa glucose regulated protein precursor 24 16 120 4.9 72 P06761
  1. a, The minimum coverage of the matched peptides in relation to the full-length sequence.
  2. b, The number of matched peptides in the database search.
  3. c, Score of the Mascot search.
  4. d, Theoretical pI of the full length protein.
  5. e, Theoretical molecular mass (Mw) of the full length protein.
  6. f, Primary accession key in the SWISS-PROT database.