PLS-DA scores plot of NMR-based metabonomic data. (A) PLS-DA scores plot from analysis of NMR-based metabonomic data obtained on extracts of control (open circles) and creatine monohydrate (CMH) treated C2C12 muscle cells (closed circles), (B) the X-loadings of the PLS-DA. The dominating signals at 3.04 and 3.94 ppm are ascribed to CH3 and CH2 in creatine, respectively. The arrow shows a signal at 2.40 ppm, which was also found to be significant in the discrimination of control and CMH-treated cells. The 2.40 ppm signal is tentatively assigned to malate.