Figure 4

Experiment demonstrating Akirin-1/Mighty mRNA knockdown affects myotube size. Legend: Data are presented as means ± standard error (n = 4–5 plates per treatment). Effects of shRNA-mediated Akirin-1/Mighty mRNA knockdown on myotube size versus a scrambled shRNA control condition (A), and confirmation that Akirin-1 mRNA was reduced in shRNA-transfected myotubes (B). Photographs in sub-figure C are 10x representative images of GFP-positive cells (arrows) that were transfected with either a scrambled (CTL)-shRNA or Akirin-1/Mighty-shRNA.