Table 4 Oligonucleotide primers for ‘Real – Time’ PCR primers
Human genes | Accession number | Forward primer | Reverse primer |
|---|---|---|---|
(5′ - 3′) | (5′ - 3′) | ||
Cyclophilin | NM_021130.3 | CATCTGCACTGCCAAGACTGA | TTCATGCCTTCTTTCACTTTGC |
GAPDH | NM_002046.3 | CAACGACCACTTTGTCAAGC | TTACTCCTTGGAGGCCATGT |
AMPK-α2 | NM_006252.3 | AACTGCAGAGAGCCATTCACTTT | GGTGAAACTGAAGACAATGTGCTT |
PGC-1α | NM_013261.3 | CAAGCCAAACCAACAACTTTATCTCT | CACACTTAAGGTGCGTTCAATAGTC |
Glycogen synthase | NM_002103.4 | GCTCCCTGTGGACTATGAGG | ATTCCCATAACCGTGCACTC |